pE8UCAT

This is a promoter cloning vector suitable for studying transcriptional activity of very weakly active promoter fragments. This was constructed by inserting a 1.025 kb SalI fragment from pUMSSB1 containing the strong transcription terminator from the mouse c-mos gene (UMS = upstream mouse sequence) into the EcoRI site of pEMBL8-CAT using EcoRI linkers. The presence of the terminator upstream of the cloning sites of the polylinker prevents transcription that starts at cryptic promoters in the vector sequence. Restriction digests of the clone give the following sizes (kb): BamHI–6.6; EcoRI–5.4, 1.1, 0.4; HindIII–6.6. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli, E.coli 71/18.