pDO17

质粒编号:
质粒名称: pDO17
其他名称:
质粒大小（bp）: 12035
抗性: Ampicillin
质粒类型: Unspecified
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测序引物:
GenBank号: U03459
其他属性: Restriction digests of the clone give the following sizes (kb): BglI–7.8, 2.5, 1.6, 0.78; EcoRI–12.5; PstI–9.0, 2.5, 1.1. (ATCC staff) Plasmid contains the following restriction sites (approximate kb from nt 1): BamHI–6.98; BglI–6.61, 7.54, 7.77, 10.9, 11.63; EcoRI–3.89; HindIII–2.31; PstI–0.65, 1.78, 5.62, 10.22; PvuI–10.34; SalI–7.26; SmaI–0.99, 4.61; XhoI–0.89. (GenBank/EMBL/DDBJ) A series of overlapping deletion segments can be generated by partial digestion with a restriction enzyme not found in the vector, followed by complete digestion with lambda terminase. The following restriction sites do not cut pDO184: AatII AflII ApaI AscI AvrII Bpu1102I Bsp120I BsrGI Bsu36I HpaI MunI NotI NsiI PacI PaeR7I PmeI PmlI Ppu10I SacII SfiI SnaBI SpeI SrfI StuI SwaI. [1] Cosmid vector containing two compatible origins of replication, for generating bidirectional deletions of large inserts to facilitate sequencing. Resulting fragments are blunted and circularized by ligation, and transformants are selected for either ampicillin or kanamycin resistance. AmpR transformants can be sequenced in the clockwise direction using the cosL primer 5'-TCATAAATAGCGAAAACC-3'. KanR transformants can be sequenced in the counterclockwise direction using the cosR primer 5'-ACTTTACGGGTCCTTTCCG-3'. [2] Growth: 1065 plus ampicillin (50 ug/ml) plus kanamycin (20 ug/ml) 30C Deposited by: Ahmed A.L. NCBI gi: 424107 Hosts: E.coli, E.coli AA102. Related vectors: pBR322, pDO2, pDO6, pDO184, pDO19.
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