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- 2025年11月21日
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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pET-21a(+)
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号: 60908-2784y
质粒名称: pET-21a(+)
其他名称:
质粒大小（bp）: 5443
抗性: Ampicillin (氨苄 青 霉 素)
质粒类型: 大肠杆菌表达载体
是否病毒: 非病毒
启动子: T7
稳转或瞬转:
组成型或诱导性:
表达水平: 高
蛋白标签: N-T7, C-His
5’测序引物:T7
5’测序引物序列:5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性:
质粒图谱:
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
| 151 | ggtggtgctc | gagtgcggcc | gcaagcttgt | cgacggagct | cgaattcgga | 200 |
| 201 | tccgcgaccc | atttgctgtc | caccagtcat | gctagccata | tgtatatctc | 250 |
| 251 | cttcttaaag | ttaaacaaaa | ttatttctag | aggggaattg | ttatccgctc | 300 |
| 301 | acaattcccc | tatagtgagt | cgtattaatt | tcgcgggatc | gagatctcga | 350 |
| 351 | tcctctacgc | cggacgcatc | gtggccggca | tcaccggcgc | cacaggtgcg | 400 |
| 401 | gttgctggcg | cctatatcgc | cgacatcacc | gatggggaag | atcgggctcg | 450 |
| 451 | ccacttcggg | ctcatgagcg | cttgtttcgg | cgtgggtatg | gtggcaggcc | 500 |
| 501 | ccgtggccgg | gggactgttg | ggcgccatct | ccttgcatgc | accattcctt | 550 |
| 551 | gcggcggcgg | tgctcaacgg | cctcaaccta | ctactgggct | gcttcctaat | 600 |
| 601 | gcaggagtcg | cataagggag | agcgtcgaga | tcccggacac | catcgaatgg | 650 |
| 651 | cgcaaaacct | ttcgcggtat | ggcatgatag | cgcccggaag | agagtcaatt | 700 |
| 701 | cagggtggtg | aatgtgaaac | cagtaacgtt | atacgatgtc | gcagagtatg | 750 |
| 751 | ccggtgtctc | ttatcagacc | gtttcccgcg | tggtgaacca | ggccagccac | 800 |
| 801 | gtttctgcga | aaacgcggga | aaaagtggaa | gcggcgatgg | cggagctgaa | 850 |
| 851 | ttacattccc | aaccgcgtgg | cacaacaact | ggcgggcaaa | cagtcgttgc | 900 |
| 901 | tgattggcgt | tgccacctcc | agtctggccc | tgcacgcgcc | gtcgcaaatt | 950 |
| 951 | gtcgcggcga | ttaaatctcg | cgccgatcaa | ctgggtgcca | gcgtggtggt | 1000 |
| 1001 | gtcgatggta | gaacgaagcg | gcgtcgaagc | ctgtaaagcg | gcggtgcaca | 1050 |
| 1051 | atcttctcgc | gcaacgcgtc | agtgggctga | tcattaacta | tccgctggat | 1100 |
| 1101 | gaccaggatg | ccattgctgt | ggaagctgcc | tgcactaatg | ttccggcgtt | 1150 |
| 1151 | atttcttgat | gtctctgacc | agacacccat | caacagtatt | attttctccc | 1200 |
| 1201 | atgaagacgg | tacgcgactg | ggcgtggagc | atctggtcgc | attgggtcac | 1250 |
| 1251 | cagcaaatcg | cgctgttagc | gggcccatta | agttctgtct | cggcgcgtct | 1300 |
| 1301 | gcgtctggct | ggctggcata | aatatctcac | tcgcaatcaa | attcagccga | 1350 |
| 1351 | tagcggaacg | ggaaggcgac | tggagtgcca | tgtccggttt | tcaacaaacc | 1400 |
| 1401 | atgcaaatgc | tgaatgaggg | catcgttccc | actgcgatgc | tggttgccaa | 1450 |
| 1451 | cgatcagatg | gcgctgggcg | caatgcgcgc | cattaccgag | tccgggctgc | 1500 |
| 1501 | gcgttggtgc | ggatatctcg | gtagtgggat | acgacgatac | cgaagacagc | 1550 |
| 1551 | tcatgttata | tcccgccgtt | aaccaccatc | aaacaggatt | ttcgcctgct | 1600 |
| 1601 | ggggcaaacc | agcgtggacc | gcttgctgca | actctctcag | ggccaggcgg | 1650 |
| 1651 | tgaagggcaa | tcagctgttg | cccgtctcac | tggtgaaaag | aaaaaccacc | 1700 |
| 1701 | ctggcgccca | atacgcaaac | cgcctctccc | cgcgcgttgg | ccgattcatt | 1750 |
| 1751 | aatgcagctg | gcacgacagg | tttcccgact | ggaaagcggg | cagtgagcgc | 1800 |
| 1801 | aacgcaatta | atgtaagtta | gctcactcat | taggcaccgg | gatctcgacc | 1850 |
| 1851 | gatgcccttg | agagccttca | acccagtcag | ctccttccgg | tgggcgcggg | 1900 |
| 1901 | gcatgactat | cgtcgccgca | cttatgactg | tcttctttat | catgcaactc | 1950 |
| 1951 | gtaggacagg | tgccggcagc | gctctgggtc | attttcggcg | aggaccgctt | 2000 |
| 2001 | tcgctggagc | gcgacgatga | tcggcctgtc | gcttgcggta | ttcggaatct | 2050 |
| 2051 | tgcacgccct | cgctcaagcc | ttcgtcactg | gtcccgccac | caaacgtttc | 2100 |
| 2101 | ggcgagaagc | aggccattat | cgccggcatg | gcggccccac | gggtgcgcat | 2150 |
| 2151 | gatcgtgctc | ctgtcgttga | ggacccggct | aggctggcgg | ggttgcctta | 2200 |
| 2201 | ctggttagca | gaatgaatca | ccgatacgcg | agcgaacgtg | aagcgactgc | 2250 |
| 2251 | tgctgcaaaa | cgtctgcgac | ctgagcaaca | acatgaatgg | tcttcggttt | 2300 |
| 2301 | ccgtgtttcg | taaagtctgg | aaacgcggaa | gtcagcgccc | tgcaccatta | 2350 |
| 2351 | tgttccggat | ctgcatcgca | ggatgctgct | ggctaccctg | tggaacacct | 2400 |
| 2401 | acatctgtat | taacgaagcg | ctggcattga | ccctgagtga | tttttctctg | 2450 |
| 2451 | gtcccgccgc | atccataccg | ccagttgttt | accctcacaa | cgttccagta | 2500 |
| 2501 | accgggcatg | ttcatcatca | gtaacccgta | tcgtgagcat | cctctctcgt | 2550 |
| 2551 | ttcatcggta | tcattacccc | catgaacaga | aatccccctt | acacggaggc | 2600 |
| 2601 | atcagtgacc | aaacaggaaa | aaaccgccct | taacatggcc | cgctttatca | 2650 |
| 2651 | gaagccagac | attaacgctt | ctggagaaac | tcaacgagct | ggacgcggat | 2700 |
| 2701 | gaacaggcag | acatctgtga | atcgcttcac | gaccacgctg | atgagcttta | 2750 |
| 2751 | ccgcagctgc | ctcgcgcgtt | tcggtgatga | cggtgaaaac | ctctgacaca | 2800 |
| 2801 | tgcagctccc | ggagacggtc | acagcttgtc | tgtaagcgga | tgccgggagc | 2850 |
| 2851 | agacaagccc | gtcagggcgc | gtcagcgggt | gttggcgggt | gtcggggcgc | 2900 |
| 2901 | agccatgacc | cagtcacgta | gcgatagcgg | agtgtatact | ggcttaacta | 2950 |
| 2951 | tgcggcatca | gagcagattg | tactgagagt | gcaccatata | tgcggtgtga | 3000 |
| 3001 | aataccgcac | agatgcgtaa | ggagaaaata | ccgcatcagg | cgctcttccg | 3050 |
| 3051 | cttcctcgct | cactgactcg | ctgcgctcgg | tcgttcggct | gcggcgagcg | 3100 |
| 3101 | gtatcagctc | actcaaaggc | ggtaatacgg | ttatccacag | aatcagggga | 3150 |
| 3151 | taacgcagga | aagaacatgt | gagcaaaagg | ccagcaaaag | gccaggaacc | 3200 |
| 3201 | gtaaaaaggc | cgcgttgctg | gcgtttttcc | ataggctccg | cccccctgac | 3250 |
| 3251 | gagcatcaca | aaaatcgacg | ctcaagtcag | aggtggcgaa | acccgacagg | 3300 |
| 3301 | actataaaga | taccaggcgt | ttccccctgg | aagctccctc | gtgcgctctc | 3350 |
| 3351 | ctgttccgac | cctgccgctt | accggatacc | tgtccgcctt | tctcccttcg | 3400 |
| 3401 | ggaagcgtgg | cgctttctca | tagctcacgc | tgtaggtatc | tcagttcggt | 3450 |
| 3451 | gtaggtcgtt | cgctccaagc | tgggctgtgt | gcacgaaccc | cccgttcagc | 3500 |
| 3501 | ccgaccgctg | cgccttatcc | ggtaactatc | gtcttgagtc | caacccggta | 3550 |
| 3551 | agacacgact | tatcgccact | ggcagcagcc | actggtaaca | ggattagcag | 3600 |
| 3601 | agcgaggtat | gtaggcggtg | ctacagagtt | cttgaagtgg | tggcctaact | 3650 |
| 3651 | acggctacac | tagaaggaca | gtatttggta | tctgcgctct | gctgaagcca | 3700 |
| 3701 | gttaccttcg | gaaaaagagt | tggtagctct | tgatccggca | aacaaaccac | 3750 |
| 3751 | cgctggtagc | ggtggttttt | ttgtttgcaa | gcagcagatt | acgcgcagaa | 3800 |
| 3801 | aaaaaggatc | tcaagaagat | cctttgatct | tttctacggg | gtctgacgct | 3850 |
| 3851 | cagtggaacg | aaaactcacg | ttaagggatt | ttggtcatga | gattatcaaa | 3900 |
| 3901 | aaggatcttc | acctagatcc | ttttaaatta | aaaatgaagt | tttaaatcaa | 3950 |
| 3951 | tctaaagtat | atatgagtaa | acttggtctg | acagttacca | atgcttaatc | 4000 |
| 4001 | agtgaggcac | ctatctcagc | gatctgtcta | tttcgttcat | ccatagttgc | 4050 |
| 4051 | ctgactcccc | gtcgtgtaga | taactacgat | acgggagggc | ttaccatctg | 4100 |
| 4101 | gccccagtgc | tgcaatgata | ccgcgagacc | cacgctcacc | ggctccagat | 4150 |
| 4151 | ttatcagcaa | taaaccagcc | agccggaagg | gccgagcgca | gaagtggtcc | 4200 |
| 4201 | tgcaacttta | tccgcctcca | tccagtctat | taattgttgc | cgggaagcta | 4250 |
| 4251 | gagtaagtag | ttcgccagtt | aatagtttgc | gcaacgttgt | tgccattgct | 4300 |
| 4301 | gcaggcatcg | tggtgtcacg | ctcgtcgttt | ggtatggctt | cattcagctc | 4350 |
| 4351 | cggttcccaa | cgatcaaggc | gagttacatg | atcccccatg | ttgtgcaaaa | 4400 |
| 4401 | aagcggttag | ctccttcggt | cctccgatcg | ttgtcagaag | taagttggcc | 4450 |
| 4451 | gcagtgttat | cactcatggt | tatggcagca | ctgcataatt | ctcttactgt | 4500 |
| 4501 | catgccatcc | gtaagatgct | tttctgtgac | tggtgagtac | tcaaccaagt | 4550 |
| 4551 | cattctgaga | atagtgtatg | cggcgaccga | gttgctcttg | cccggcgtca | 4600 |
| 4601 | atacgggata | ataccgcgcc | acatagcaga | actttaaaag | tgctcatcat | 4650 |
| 4651 | tggaaaacgt | tcttcggggc | gaaaactctc | aaggatctta | ccgctgttga | 4700 |
| 4701 | gatccagttc | gatgtaaccc | actcgtgcac | ccaactgatc | ttcagcatct | 4750 |
| 4751 | tttactttca | ccagcgtttc | tgggtgagca | aaaacaggaa | ggcaaaatgc | 4800 |
| 4801 | cgcaaaaaag | ggaataaggg | cgacacggaa | atgttgaata | ctcatactct | 4850 |
| 4851 | tcctttttca | atattattga | agcatttatc | agggttattg | tctcatgagc | 4900 |
| 4901 | ggatacatat | ttgaatgtat | ttagaaaaat | aaacaaatag | gggttccgcg | 4950 |
| 4951 | cacatttccc | cgaaaagtgc | cacctgaaat | tgtaaacgtt | aatattttgt | 5000 |
| 5001 | taaaattcgc | gttaaatttt | tgttaaatca | gctcattttt | taaccaatag | 5050 |
| 5051 | gccgaaatcg | gcaaaatccc | ttataaatca | aaagaataga | ccgagatagg | 5100 |
| 5101 | gttgagtgtt | gttccagttt | ggaacaagag | tccactatta | aagaacgtgg | 5150 |
| 5151 | actccaacgt | caaagggcga | aaaaccgtct | atcagggcga | tggcccacta | 5200 |
| 5201 | cgtgaaccat | caccctaatc | aagttttttg | gggtcgaggt | gccgtaaagc | 5250 |
| 5251 | actaaatcgg | aaccctaaag | ggagcccccg | atttagagct | tgacggggaa | 5300 |
| 5301 | agccggcgaa | cgtggcgaga | aaggaaggga | agaaagcgaa | aggagcgggc | 5350 |
| 5351 | gctagggcgc | tggcaagtgt | agcggtcacg | ctgcgcgtaa | ccaccacacc | 5400 |
| 5401 | cgccgcgctt | aatgcgccgc | tacagggcgc | gtcccattcg | cca | 5443 |
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pET-21a(+)
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