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- 2025年11月20日
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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pBIIOI
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号:
质粒名称: pBIIOI
其他名称:
质粒大小（bp）: 4373
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性: Created by Moore, July 1995, under contract with NCBI. General purpose cloning vector. (ATCC staff) Derivative of pBR322 with a BglII linker inserted at the EcoRI site. (personal communication) The order of the major features in this plasmid is:EcoRI – BglII – EcoRI – ClaI – HindIII – tet – PvuII – ori – bla. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI–4.4; BglII–4.4; EcoRI/PstI–3.8, 0.7; HindIII–4.4; EcoRI/PvuII–2.3, 2.1. (ATCC staff) Medium is 1315 1065 plus ampicillin (100 ug/ul). Hosts: E.coli RR1, E.coli. Related vectors: pBR322.
质粒图谱:
质粒序列:
| 1 | ttctcatgtt | tgacagctta | tcatcgataa | gctttaatgc | ggtagtttat | 50 |
| 51 | cacagttaaa | ttgctaacgc | agtcaggcac | cgtgtatgaa | atctaacaat | 100 |
| 101 | gcgctcatcg | tcatcctcgg | caccgtcacc | ctggatgctg | taggcatagg | 150 |
| 151 | cttggttatg | ccggtactgc | cgggcctctt | gcgggatatc | gtccattccg | 200 |
| 201 | acagcatcgc | cagtcactat | ggcgtgctgc | tagcgctata | tgcgttgatg | 250 |
| 251 | caatttctat | gcgcacccgt | tctcggagca | ctgtccgacc | gctttggccg | 300 |
| 301 | ccgcccagtc | ctgctcgctt | cgctacttgg | agccactatc | gactacgcga | 350 |
| 351 | tcatggcgac | cacacccgtc | ctgtggatcc | tctacgccgg | acgcatcgtg | 400 |
| 401 | gccggcatca | ccggcgccac | aggtgcggtt | gctggcgcct | atatcgccga | 450 |
| 451 | catcaccgat | ggggaagatc | gggctcgcca | cttcgggctc | atgagcgctt | 500 |
| 501 | gtttcggcgt | gggtatggtg | gcaggccccg | tggccggggg | actgttgggc | 550 |
| 551 | gccatctcct | tgcatgcacc | attccttgcg | gcggcggtgc | tcaacggcct | 600 |
| 601 | caacctacta | ctgggctgct | tcctaatgca | ggagtcgcat | aagggagagc | 650 |
| 651 | gtcgaccgat | gcccttgaga | gccttcaacc | cagtcagctc | cttccggtgg | 700 |
| 701 | gcgcggggca | tgactatcgt | cgccgcactt | atgactgtct | tctttatcat | 750 |
| 751 | gcaactcgta | ggacaggtgc | cggcagcgct | ctgggtcatt | ttcggcgagg | 800 |
| 801 | accgctttcg | ctggagcgcg | acgatgatcg | gcctgtcgct | tgcggtattc | 850 |
| 851 | ggaatcttgc | acgccctcgc | tcaagccttc | gtcactggtc | ccgccaccaa | 900 |
| 901 | acgtttcggc | gagaagcagg | ccattatcgc | cggcatggcg | gccgacgcgc | 950 |
| 951 | tgggctacgt | cttgctggcg | ttcgcgacgc | gaggctggat | ggccttcccc | 1000 |
| 1001 | attatgattc | ttctcgcttc | cggcggcatc | gggatgcccg | cgttgcaggc | 1050 |
| 1051 | catgctgtcc | aggcaggtag | atgacgacca | tcagggacag | cttcaaggat | 1100 |
| 1101 | cgctcgcggc | tcttaccagc | ctaacttcga | tcactggacc | gctgatcgtc | 1150 |
| 1151 | acggcgattt | atgccgcctc | ggcgagcaca | tggaacgggt | tggcatggat | 1200 |
| 1201 | tgtaggcgcc | gccctatacc | ttgtctgcct | ccccgcgttg | cgtcgcggtg | 1250 |
| 1251 | catggagccg | ggccacctcg | acctgaatgg | aagccggcgg | cacctcgcta | 1300 |
| 1301 | acggattcac | cactccaaga | attggagcca | atcaattctt | gcggagaact | 1350 |
| 1351 | gtgaatgcgc | aaaccaaccc | ttggcagaac | atatccatcg | cgtccgccat | 1400 |
| 1401 | ctccagcagc | cgcacgcggc | gcatctcggg | cagcgttggg | tcctggccac | 1450 |
| 1451 | gggtgcgcat | gatcgtgctc | ctgtcgttga | ggacccggct | aggctggcgg | 1500 |
| 1501 | ggttgcctta | ctggttagca | gaatgaatca | ccgatacgcg | agcgaacgtg | 1550 |
| 1551 | aagcgactgc | tgctgcaaaa | cgtctgcgac | ctgagcaaca | acatgaatgg | 1600 |
| 1601 | tcttcggttt | ccgtgtttcg | taaagtctgg | aaacgcggaa | gtcagcgccc | 1650 |
| 1651 | tgcaccatta | tgttccggat | ctgcatcgca | ggatgctgct | ggctaccctg | 1700 |
| 1701 | tggaacacct | acatctgtat | taacgaagcg | ctggcattga | ccctgagtga | 1750 |
| 1751 | tttttctctg | gtcccgccgc | atccataccg | ccagttgttt | accctcacaa | 1800 |
| 1801 | cgttccagta | accgggcatg | ttcatcatca | gtaacccgta | tcgtgagcat | 1850 |
| 1851 | cctctctcgt | ttcatcggta | tcattacccc | catgaacaga | aatccccctt | 1900 |
| 1901 | acacggaggc | atcagtgacc | aaacaggaaa | aaaccgccct | taacatggcc | 1950 |
| 1951 | cgctttatca | gaagccagac | attaacgctt | ctggagaaac | tcaacgagct | 2000 |
| 2001 | ggacgcggat | gaacaggcag | acatctgtga | atcgcttcac | gaccacgctg | 2050 |
| 2051 | atgagcttta | ccgcagctgc | ctcgcgcgtt | tcggtgatga | cggtgaaaac | 2100 |
| 2101 | ctctgacaca | tgcagctccc | ggagacggtc | acagcttgtc | tgtaagcgga | 2150 |
| 2151 | tgccgggagc | agacaagccc | gtcagggcgc | gtcagcgggt | gttggcgggt | 2200 |
| 2201 | gtcggggcgc | agccatgacc | cagtcacgta | gcgatagcgg | agtgtatact | 2250 |
| 2251 | ggcttaacta | tgcggcatca | gagcagattg | tactgagagt | gcaccatatg | 2300 |
| 2301 | cggtgtgaaa | taccgcacag | atgcgtaagg | agaaaatacc | gcatcaggcg | 2350 |
| 2351 | ctcttccgct | tcctcgctca | ctgactcgct | gcgctcggtc | gttcggctgc | 2400 |
| 2401 | ggcgagcggt | atcagctcac | tcaaaggcgg | taatacggtt | atccacagaa | 2450 |
| 2451 | tcaggggata | acgcaggaaa | gaacatgtga | gcaaaaggcc | agcaaaaggc | 2500 |
| 2501 | caggaaccgt | aaaaaggccg | cgttgctggc | gtttttccat | aggctccgcc | 2550 |
| 2551 | cccctgacga | gcatcacaaa | aatcgacgct | caagtcagag | gtggcgaaac | 2600 |
| 2601 | ccgacaggac | tataaagata | ccaggcgttt | ccccctggaa | gctccctcgt | 2650 |
| 2651 | gcgctctcct | gttccgaccc | tgccgcttac | cggatacctg | tccgcctttc | 2700 |
| 2701 | tcccttcggg | aagcgtggcg | ctttctcata | gctcacgctg | taggtatctc | 2750 |
| 2751 | agttcggtgt | aggtcgttcg | ctccaagctg | ggctgtgtgc | acgaaccccc | 2800 |
| 2801 | cgttcagccc | gaccgctgcg | ccttatccgg | taactatcgt | cttgagtcca | 2850 |
| 2851 | acccggtaag | acacgactta | tcgccactgg | cagcagccac | tggtaacagg | 2900 |
| 2901 | attagcagag | cgaggtatgt | aggcggtgct | acagagttct | tgaagtggtg | 2950 |
| 2951 | gcctaactac | ggctacacta | gaaggacagt | atttggtatc | tgcgctctgc | 3000 |
| 3001 | tgaagccagt | taccttcgga | aaaagagttg | gtagctcttg | atccggcaaa | 3050 |
| 3051 | caaaccaccg | ctggtagcgg | tggttttttt | gtttgcaagc | agcagattac | 3100 |
| 3101 | gcgcagaaaa | aaaggatctc | aagaagatcc | tttgatcttt | tctacggggt | 3150 |
| 3151 | ctgacgctca | gtggaacgaa | aactcacgtt | aagggatttt | ggtcatgaga | 3200 |
| 3201 | ttatcaaaaa | ggatcttcac | ctagatcctt | ttaaattaaa | aatgaagttt | 3250 |
| 3251 | taaatcaatc | taaagtatat | atgagtaaac | ttggtctgac | agttaccaat | 3300 |
| 3301 | gcttaatcag | tgaggcacct | atctcagcga | tctgtctatt | tcgttcatcc | 3350 |
| 3351 | atagttgcct | gactccccgt | cgtgtagata | actacgatac | gggagggctt | 3400 |
| 3401 | accatctggc | cccagtgctg | caatgatacc | gcgagaccca | cgctcaccgg | 3450 |
| 3451 | ctccagattt | atcagcaata | aaccagccag | ccggaagggc | cgagcgcaga | 3500 |
| 3501 | agtggtcctg | caactttatc | cgcctccatc | cagtctatta | attgttgccg | 3550 |
| 3551 | ggaagctaga | gtaagtagtt | cgccagttaa | tagtttgcgc | aacgttgttg | 3600 |
| 3601 | ccattgctgc | aggcatcgtg | gtgtcacgct | cgtcgtttgg | tatggcttca | 3650 |
| 3651 | ttcagctccg | gttcccaacg | atcaaggcga | gttacatgat | cccccatgtt | 3700 |
| 3701 | gtgcaaaaaa | gcggttagct | ccttcggtcc | tccgatcgtt | gtcagaagta | 3750 |
| 3751 | agttggccgc | agtgttatca | ctcatggtta | tggcagcact | gcataattct | 3800 |
| 3801 | cttactgtca | tgccatccgt | aagatgcttt | tctgtgactg | gtgagtactc | 3850 |
| 3851 | aaccaagtca | ttctgagaat | agtgtatgcg | gcgaccgagt | tgctcttgcc | 3900 |
| 3901 | cggcgtcaac | acgggataat | accgcgccac | atagcagaac | tttaaaagtg | 3950 |
| 3951 | ctcatcattg | gaaaacgttc | ttcggggcga | aaactctcaa | ggatcttacc | 4000 |
| 4001 | gctgttgaga | tccagttcga | tgtaacccac | tcgtgcaccc | aactgatctt | 4050 |
| 4051 | cagcatcttt | tactttcacc | agcgtttctg | ggtgagcaaa | aacaggaagg | 4100 |
| 4101 | caaaatgccg | caaaaaaggg | aataagggcg | acacggaaat | gttgaatact | 4150 |
| 4151 | catactcttc | ctttttcaat | attattgaag | catttatcag | ggttattgtc | 4200 |
| 4201 | tcatgagcgg | atacatattt | gaatgtattt | agaaaaataa | acaaataggg | 4250 |
| 4251 | gttccgcgca | catttccccg | aaaagtgcca | cctgacgtct | aagaaaccat | 4300 |
| 4301 | tattatcatg | acattaacct | ataaaaatag | gcgtatcacg | aggccctttc | 4350 |
| 4351 | gtcttcaaga | attcagatct | gaa | 4373 |
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pBIIOI
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