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- TIANDZ
- ZK-0023706
- 中国
- 2025年11月21日
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![pGL4.23[luc2 minP]](https://img1.dxycdn.com/p/s14/2025/1023/964/4057766217249821891.jpg!wh200)
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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pET-29a(+)
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号: 60908-2794y
质粒名称: pET-29a(+)
其他名称:
质粒大小（bp）: 5371
抗性: Kanamycin (卡那霉素)
质粒类型: 大肠杆菌表达载体
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签: N-S, N-Thrombin, C-His
5′ 测序引物:T7
5′ 测序引物序列: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性:
质粒图谱:
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
| 151 | ggtggtgctc | gagtgcggcc | gcaagcttgt | cgacggagct | cgaattcgga | 200 |
| 201 | tccgatatca | gccatggaac | cgcgtggcac | cagggtaccc | agatctgggc | 250 |
| 251 | tgtccatgtg | ctggcgttcg | aatttagcag | cagcggtttc | tttcatatgt | 300 |
| 301 | atatctcctt | cttaaagtta | aacaaaatta | tttctagagg | ggaattgtta | 350 |
| 351 | tccgctcaca | attcccctat | agtgagtcgt | attaatttcg | cgggatcgag | 400 |
| 401 | atcgatctcg | atcctctacg | ccggacgcat | cgtggccggc | atcaccggcg | 450 |
| 451 | ccacaggtgc | ggttgctggc | gcctatatcg | ccgacatcac | cgatggggaa | 500 |
| 501 | gatcgggctc | gccacttcgg | gctcatgagc | gcttgtttcg | gcgtgggtat | 550 |
| 551 | ggtggcaggc | cccgtggccg | ggggactgtt | gggcgccatc | tccttgcatg | 600 |
| 601 | caccattcct | tgcggcggcg | gtgctcaacg | gcctcaacct | actactgggc | 650 |
| 651 | tgcttcctaa | tgcaggagtc | gcataaggga | gagcgtcgag | atcccggaca | 700 |
| 701 | ccatcgaatg | gcgcaaaacc | tttcgcggta | tggcatgata | gcgcccggaa | 750 |
| 751 | gagagtcaat | tcagggtggt | gaatgtgaaa | ccagtaacgt | tatacgatgt | 800 |
| 801 | cgcagagtat | gccggtgtct | cttatcagac | cgtttcccgc | gtggtgaacc | 850 |
| 851 | aggccagcca | cgtttctgcg | aaaacgcggg | aaaaagtgga | agcggcgatg | 900 |
| 901 | gcggagctga | attacattcc | caaccgcgtg | gcacaacaac | tggcgggcaa | 950 |
| 951 | acagtcgttg | ctgattggcg | ttgccacctc | cagtctggcc | ctgcacgcgc | 1000 |
| 1001 | cgtcgcaaat | tgtcgcggcg | attaaatctc | gcgccgatca | actgggtgcc | 1050 |
| 1051 | agcgtggtgg | tgtcgatggt | agaacgaagc | ggcgtcgaag | cctgtaaagc | 1100 |
| 1101 | ggcggtgcac | aatcttctcg | cgcaacgcgt | cagtgggctg | atcattaact | 1150 |
| 1151 | atccgctgga | tgaccaggat | gccattgctg | tggaagctgc | ctgcactaat | 1200 |
| 1201 | gttccggcgt | tatttcttga | tgtctctgac | cagacaccca | tcaacagtat | 1250 |
| 1251 | tattttctcc | catgaagacg | gtacgcgact | gggcgtggag | catctggtcg | 1300 |
| 1301 | cattgggtca | ccagcaaatc | gcgctgttag | cgggcccatt | aagttctgtc | 1350 |
| 1351 | tcggcgcgtc | tgcgtctggc | tggctggcat | aaatatctca | ctcgcaatca | 1400 |
| 1401 | aattcagccg | atagcggaac | gggaaggcga | ctggagtgcc | atgtccggtt | 1450 |
| 1451 | ttcaacaaac | catgcaaatg | ctgaatgagg | gcatcgttcc | cactgcgatg | 1500 |
| 1501 | ctggttgcca | acgatcagat | ggcgctgggc | gcaatgcgcg | ccattaccga | 1550 |
| 1551 | gtccgggctg | cgcgttggtg | cggacatctc | ggtagtggga | tacgacgata | 1600 |
| 1601 | ccgaagacag | ctcatgttat | atcccgccgt | taaccaccat | caaacaggat | 1650 |
| 1651 | tttcgcctgc | tggggcaaac | cagcgtggac | cgcttgctgc | aactctctca | 1700 |
| 1701 | gggccaggcg | gtgaagggca | atcagctgtt | gcccgtctca | ctggtgaaaa | 1750 |
| 1751 | gaaaaaccac | cctggcgccc | aatacgcaaa | ccgcctctcc | ccgcgcgttg | 1800 |
| 1801 | gccgattcat | taatgcagct | ggcacgacag | gtttcccgac | tggaaagcgg | 1850 |
| 1851 | gcagtgagcg | caacgcaatt | aatgtaagtt | agctcactca | ttaggcaccg | 1900 |
| 1901 | ggatctcgac | cgatgccctt | gagagccttc | aacccagtca | gctccttccg | 1950 |
| 1951 | gtgggcgcgg | ggcatgacta | tcgtcgccgc | acttatgact | gtcttcttta | 2000 |
| 2001 | tcatgcaact | cgtaggacag | gtgccggcag | cgctctgggt | cattttcggc | 2050 |
| 2051 | gaggaccgct | ttcgctggag | cgcgacgatg | atcggcctgt | cgcttgcggt | 2100 |
| 2101 | attcggaatc | ttgcacgccc | tcgctcaagc | cttcgtcact | ggtcccgcca | 2150 |
| 2151 | ccaaacgttt | cggcgagaag | caggccatta | tcgccggcat | ggcggcccca | 2200 |
| 2201 | cgggtgcgca | tgatcgtgct | cctgtcgttg | aggacccggc | taggctggcg | 2250 |
| 2251 | gggttgcctt | actggttagc | agaatgaatc | accgatacgc | gagcgaacgt | 2300 |
| 2301 | gaagcgactg | ctgctgcaaa | acgtctgcga | cctgagcaac | aacatgaatg | 2350 |
| 2351 | gtcttcggtt | tccgtgtttc | gtaaagtctg | gaaacgcgga | agtcagcgcc | 2400 |
| 2401 | ctgcaccatt | atgttccgga | tctgcatcgc | aggatgctgc | tggctaccct | 2450 |
| 2451 | gtggaacacc | tacatctgta | ttaacgaagc | gctggcattg | accctgagtg | 2500 |
| 2501 | atttttctct | ggtcccgccg | catccatacc | gccagttgtt | taccctcaca | 2550 |
| 2551 | acgttccagt | aaccgggcat | gttcatcatc | agtaacccgt | atcgtgagca | 2600 |
| 2601 | tcctctctcg | tttcatcggt | atcattaccc | ccatgaacag | aaatccccct | 2650 |
| 2651 | tacacggagg | catcagtgac | caaacaggaa | aaaaccgccc | ttaacatggc | 2700 |
| 2701 | ccgctttatc | agaagccaga | cattaacgct | tctggagaaa | ctcaacgagc | 2750 |
| 2751 | tggacgcgga | tgaacaggca | gacatctgtg | aatcgcttca | cgaccacgct | 2800 |
| 2801 | gatgagcttt | accgcagctg | cctcgcgcgt | ttcggtgatg | acggtgaaaa | 2850 |
| 2851 | cctctgacac | atgcagctcc | cggagacggt | cacagcttgt | ctgtaagcgg | 2900 |
| 2901 | atgccgggag | cagacaagcc | cgtcagggcg | cgtcagcggg | tgttggcggg | 2950 |
| 2951 | tgtcggggcg | cagccatgac | ccagtcacgt | agcgatagcg | gagtgtatac | 3000 |
| 3001 | tggcttaact | atgcggcatc | agagcagatt | gtactgagag | tgcaccatat | 3050 |
| 3051 | atgcggtgtg | aaataccgca | cagatgcgta | aggagaaaat | accgcatcag | 3100 |
| 3101 | gcgctcttcc | gcttcctcgc | tcactgactc | gctgcgctcg | gtcgttcggc | 3150 |
| 3151 | tgcggcgagc | ggtatcagct | cactcaaagg | cggtaatacg | gttatccaca | 3200 |
| 3201 | gaatcagggg | ataacgcagg | aaagaacatg | tgagcaaaag | gccagcaaaa | 3250 |
| 3251 | ggccaggaac | cgtaaaaagg | ccgcgttgct | ggcgtttttc | cataggctcc | 3300 |
| 3301 | gcccccctga | cgagcatcac | aaaaatcgac | gctcaagtca | gaggtggcga | 3350 |
| 3351 | aacccgacag | gactataaag | ataccaggcg | tttccccctg | gaagctccct | 3400 |
| 3401 | cgtgcgctct | cctgttccga | ccctgccgct | taccggatac | ctgtccgcct | 3450 |
| 3451 | ttctcccttc | gggaagcgtg | gcgctttctc | atagctcacg | ctgtaggtat | 3500 |
| 3501 | ctcagttcgg | tgtaggtcgt | tcgctccaag | ctgggctgtg | tgcacgaacc | 3550 |
| 3551 | ccccgttcag | cccgaccgct | gcgccttatc | cggtaactat | cgtcttgagt | 3600 |
| 3601 | ccaacccggt | aagacacgac | ttatcgccac | tggcagcagc | cactggtaac | 3650 |
| 3651 | aggattagca | gagcgaggta | tgtaggcggt | gctacagagt | tcttgaagtg | 3700 |
| 3701 | gtggcctaac | tacggctaca | ctagaaggac | agtatttggt | atctgcgctc | 3750 |
| 3751 | tgctgaagcc | agttaccttc | ggaaaaagag | ttggtagctc | ttgatccggc | 3800 |
| 3801 | aaacaaacca | ccgctggtag | cggtggtttt | tttgtttgca | agcagcagat | 3850 |
| 3851 | tacgcgcaga | aaaaaaggat | ctcaagaaga | tcctttgatc | ttttctacgg | 3900 |
| 3901 | ggtctgacgc | tcagtggaac | gaaaactcac | gttaagggat | tttggtcatg | 3950 |
| 3951 | aacaataaaa | ctgtctgctt | acataaacag | taatacaagg | ggtgttatga | 4000 |
| 4001 | gccatattca | acgggaaacg | tcttgctcta | ggccgcgatt | aaattccaac | 4050 |
| 4051 | atggatgctg | atttatatgg | gtataaatgg | gctcgcgata | atgtcgggca | 4100 |
| 4101 | atcaggtgcg | acaatctatc | gattgtatgg | gaagcccgat | gcgccagagt | 4150 |
| 4151 | tgtttctgaa | acatggcaaa | ggtagcgttg | ccaatgatgt | tacagatgag | 4200 |
| 4201 | atggtcagac | taaactggct | gacggaattt | atgcctcttc | cgaccatcaa | 4250 |
| 4251 | gcattttatc | cgtactcctg | atgatgcatg | gttactcacc | actgcgatcc | 4300 |
| 4301 | ccgggaaaac | agcattccag | gtattagaag | aatatcctga | ttcaggtgaa | 4350 |
| 4351 | aatattgttg | atgcgctggc | agtgttcctg | cgccggttgc | attcgattcc | 4400 |
| 4401 | tgtttgtaat | tgtcctttta | acagcgatcg | cgtatttcgt | ctcgctcagg | 4450 |
| 4451 | cgcaatcacg | aatgaataac | ggtttggttg | atgcgagtga | ttttgatgac | 4500 |
| 4501 | gagcgtaatg | gctggcctgt | tgaacaagtc | tggaaagaaa | tgcataaact | 4550 |
| 4551 | tttgccattc | tcaccggatt | cagtcgtcac | tcatggtgat | ttctcacttg | 4600 |
| 4601 | ataaccttat | ttttgacgag | gggaaattaa | taggttgtat | tgatgttgga | 4650 |
| 4651 | cgagtcggaa | tcgcagaccg | ataccaggat | cttgccatcc | tatggaactg | 4700 |
| 4701 | cctcggtgag | ttttctcctt | cattacagaa | acggcttttt | caaaaatatg | 4750 |
| 4751 | gtattgataa | tcctgatatg | aataaattgc | agtttcattt | gatgctcgat | 4800 |
| 4801 | gagtttttct | aagaattaat | tcatgagcgg | atacatattt | gaatgtattt | 4850 |
| 4851 | agaaaaataa | acaaataggg | gttccgcgca | catttccccg | aaaagtgcca | 4900 |
| 4901 | cctgaaattg | taaacgttaa | tattttgtta | aaattcgcgt | taaatttttg | 4950 |
| 4951 | ttaaatcagc | tcatttttta | accaataggc | cgaaatcggc | aaaatccctt | 5000 |
| 5001 | ataaatcaaa | agaatagacc | gagatagggt | tgagtgttgt | tccagtttgg | 5050 |
| 5051 | aacaagagtc | cactattaaa | gaacgtggac | tccaacgtca | aagggcgaaa | 5100 |
| 5101 | aaccgtctat | cagggcgatg | gcccactacg | tgaaccatca | ccctaatcaa | 5150 |
| 5151 | gttttttggg | gtcgaggtgc | cgtaaagcac | taaatcggaa | ccctaaaggg | 5200 |
| 5201 | agcccccgat | ttagagcttg | acggggaaag | ccggcgaacg | tggcgagaaa | 5250 |
| 5251 | ggaagggaag | aaagcgaaag | gagcgggcgc | tagggcgctg | gcaagtgtag | 5300 |
| 5301 | cggtcacgct | gcgcgtaacc | accacacccg | ccgcgcttaa | tgcgccgcta | 5350 |
| 5351 | cagggcgcgt | cccattcgcc | a | 5371 |
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pET-29a(+)
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