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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pET-30b(+)
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号:
质粒名称: pET-30b(+)
其他名称: pET30b, pET 30b
质粒大小（bp）: 5421
抗性: Kanamycin (卡那霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: 高
蛋白标签: N-His, N-S, N-Thrombin, N-EK, C-His
5′ 测序引物:T7
5′ 测序引物序列: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: Nterm thrombin cleavage site; Nterm enterokinase cleavage site; a,b,c vary by MCS
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 129 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| EK | 229 | 218 | |
| lacI | 301 | 1907 | |
| T7_transl_en_RBS | 371 | 355 | |
| lacO | 416 | 389 | |
| T7_promoter | 434 | 416 | |
| tet (300 – 563) | 470 | 733 | |
| pBRrevBam_primer | 541 | 522 | |
| tet (576 – 851) | 1966 | 2241 | |
| ROP | 2716 | 2907 | |
| pGEX_3_primer | 2923 | 2901 | |
| pBR322_origin | 3941 | 3322 | |
| KanR2 | 4047 | 4862 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 2 | 326 | 1075 | |
| ORF frame 3 | 948 | 1907 | |
| ORF frame 3 | 4047 | 4862 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 158 |
| NotI | 166 |
| EagI | 166 |
| HindIII | 173 |
| SalI | 179 |
| SacI | 190 |
| EcoRI | 192 |
| BamHI | 198 |
| EcoRV | 206 |
| NcoI | 211 |
| KpnI | 237 |
| BglII | 240 |
| BstBI | 267 |
| NdeI | 345 |
| XbaI | 383 |
| BclI | 1189 |
| ApaI | 1386 |
| HpaI | 1681 |
| FspI | 2257 |
| NruI | 4135 |
| SmaI | 4352 |
| XmaI | 4350 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
| 151 | ggtggtgctc | gagtgcggcc | gcaagcttgt | cgacggagct | cgaattcgga | 200 |
| 201 | tccgatatcg | ccatggcctt | gtcgtcgtcg | tcggtaccca | gatctgggct | 250 |
| 251 | gtccatgtgc | tggcgttcga | atttagcagc | agcggtttct | ttcataccag | 300 |
| 301 | aaccgcgtgg | caccagacca | gaagaatgat | gatgatgatg | gtgcatatgt | 350 |
| 351 | atatctcctt | cttaaagtta | aacaaaatta | tttctagagg | ggaattgtta | 400 |
| 401 | tccgctcaca | attcccctat | agtgagtcgt | attaatttcg | cgggatcgag | 450 |
| 451 | atcgatctcg | atcctctacg | ccggacgcat | cgtggccggc | atcaccggcg | 500 |
| 501 | ccacaggtgc | ggttgctggc | gcctatatcg | ccgacatcac | cgatggggaa | 550 |
| 551 | gatcgggctc | gccacttcgg | gctcatgagc | gcttgtttcg | gcgtgggtat | 600 |
| 601 | ggtggcaggc | cccgtggccg | ggggactgtt | gggcgccatc | tccttgcatg | 650 |
| 651 | caccattcct | tgcggcggcg | gtgctcaacg | gcctcaacct | actactgggc | 700 |
| 701 | tgcttcctaa | tgcaggagtc | gcataaggga | gagcgtcgag | atcccggaca | 750 |
| 751 | ccatcgaatg | gcgcaaaacc | tttcgcggta | tggcatgata | gcgcccggaa | 800 |
| 801 | gagagtcaat | tcagggtggt | gaatgtgaaa | ccagtaacgt | tatacgatgt | 850 |
| 851 | cgcagagtat | gccggtgtct | cttatcagac | cgtttcccgc | gtggtgaacc | 900 |
| 901 | aggccagcca | cgtttctgcg | aaaacgcggg | aaaaagtgga | agcggcgatg | 950 |
| 951 | gcggagctga | attacattcc | caaccgcgtg | gcacaacaac | tggcgggcaa | 1000 |
| 1001 | acagtcgttg | ctgattggcg | ttgccacctc | cagtctggcc | ctgcacgcgc | 1050 |
| 1051 | cgtcgcaaat | tgtcgcggcg | attaaatctc | gcgccgatca | actgggtgcc | 1100 |
| 1101 | agcgtggtgg | tgtcgatggt | agaacgaagc | ggcgtcgaag | cctgtaaagc | 1150 |
| 1151 | ggcggtgcac | aatcttctcg | cgcaacgcgt | cagtgggctg | atcattaact | 1200 |
| 1201 | atccgctgga | tgaccaggat | gccattgctg | tggaagctgc | ctgcactaat | 1250 |
| 1251 | gttccggcgt | tatttcttga | tgtctctgac | cagacaccca | tcaacagtat | 1300 |
| 1301 | tattttctcc | catgaagacg | gtacgcgact | gggcgtggag | catctggtcg | 1350 |
| 1351 | cattgggtca | ccagcaaatc | gcgctgttag | cgggcccatt | aagttctgtc | 1400 |
| 1401 | tcggcgcgtc | tgcgtctggc | tggctggcat | aaatatctca | ctcgcaatca | 1450 |
| 1451 | aattcagccg | atagcggaac | gggaaggcga | ctggagtgcc | atgtccggtt | 1500 |
| 1501 | ttcaacaaac | catgcaaatg | ctgaatgagg | gcatcgttcc | cactgcgatg | 1550 |
| 1551 | ctggttgcca | acgatcagat | ggcgctgggc | gcaatgcgcg | ccattaccga | 1600 |
| 1601 | gtccgggctg | cgcgttggtg | cggacatctc | ggtagtggga | tacgacgata | 1650 |
| 1651 | ccgaagacag | ctcatgttat | atcccgccgt | taaccaccat | caaacaggat | 1700 |
| 1701 | tttcgcctgc | tggggcaaac | cagcgtggac | cgcttgctgc | aactctctca | 1750 |
| 1751 | gggccaggcg | gtgaagggca | atcagctgtt | gcccgtctca | ctggtgaaaa | 1800 |
| 1801 | gaaaaaccac | cctggcgccc | aatacgcaaa | ccgcctctcc | ccgcgcgttg | 1850 |
| 1851 | gccgattcat | taatgcagct | ggcacgacag | gtttcccgac | tggaaagcgg | 1900 |
| 1901 | gcagtgagcg | caacgcaatt | aatgtaagtt | agctcactca | ttaggcaccg | 1950 |
| 1951 | ggatctcgac | cgatgccctt | gagagccttc | aacccagtca | gctccttccg | 2000 |
| 2001 | gtgggcgcgg | ggcatgacta | tcgtcgccgc | acttatgact | gtcttcttta | 2050 |
| 2051 | tcatgcaact | cgtaggacag | gtgccggcag | cgctctgggt | cattttcggc | 2100 |
| 2101 | gaggaccgct | ttcgctggag | cgcgacgatg | atcggcctgt | cgcttgcggt | 2150 |
| 2151 | attcggaatc | ttgcacgccc | tcgctcaagc | cttcgtcact | ggtcccgcca | 2200 |
| 2201 | ccaaacgttt | cggcgagaag | caggccatta | tcgccggcat | ggcggcccca | 2250 |
| 2251 | cgggtgcgca | tgatcgtgct | cctgtcgttg | aggacccggc | taggctggcg | 2300 |
| 2301 | gggttgcctt | actggttagc | agaatgaatc | accgatacgc | gagcgaacgt | 2350 |
| 2351 | gaagcgactg | ctgctgcaaa | acgtctgcga | cctgagcaac | aacatgaatg | 2400 |
| 2401 | gtcttcggtt | tccgtgtttc | gtaaagtctg | gaaacgcgga | agtcagcgcc | 2450 |
| 2451 | ctgcaccatt | atgttccgga | tctgcatcgc | aggatgctgc | tggctaccct | 2500 |
| 2501 | gtggaacacc | tacatctgta | ttaacgaagc | gctggcattg | accctgagtg | 2550 |
| 2551 | atttttctct | ggtcccgccg | catccatacc | gccagttgtt | taccctcaca | 2600 |
| 2601 | acgttccagt | aaccgggcat | gttcatcatc | agtaacccgt | atcgtgagca | 2650 |
| 2651 | tcctctctcg | tttcatcggt | atcattaccc | ccatgaacag | aaatccccct | 2700 |
| 2701 | tacacggagg | catcagtgac | caaacaggaa | aaaaccgccc | ttaacatggc | 2750 |
| 2751 | ccgctttatc | agaagccaga | cattaacgct | tctggagaaa | ctcaacgagc | 2800 |
| 2801 | tggacgcgga | tgaacaggca | gacatctgtg | aatcgcttca | cgaccacgct | 2850 |
| 2851 | gatgagcttt | accgcagctg | cctcgcgcgt | ttcggtgatg | acggtgaaaa | 2900 |
| 2901 | cctctgacac | atgcagctcc | cggagacggt | cacagcttgt | ctgtaagcgg | 2950 |
| 2951 | atgccgggag | cagacaagcc | cgtcagggcg | cgtcagcggg | tgttggcggg | 3000 |
| 3001 | tgtcggggcg | cagccatgac | ccagtcacgt | agcgatagcg | gagtgtatac | 3050 |
| 3051 | tggcttaact | atgcggcatc | agagcagatt | gtactgagag | tgcaccatat | 3100 |
| 3101 | atgcggtgtg | aaataccgca | cagatgcgta | aggagaaaat | accgcatcag | 3150 |
| 3151 | gcgctcttcc | gcttcctcgc | tcactgactc | gctgcgctcg | gtcgttcggc | 3200 |
| 3201 | tgcggcgagc | ggtatcagct | cactcaaagg | cggtaatacg | gttatccaca | 3250 |
| 3251 | gaatcagggg | ataacgcagg | aaagaacatg | tgagcaaaag | gccagcaaaa | 3300 |
| 3301 | ggccaggaac | cgtaaaaagg | ccgcgttgct | ggcgtttttc | cataggctcc | 3350 |
| 3351 | gcccccctga | cgagcatcac | aaaaatcgac | gctcaagtca | gaggtggcga | 3400 |
| 3401 | aacccgacag | gactataaag | ataccaggcg | tttccccctg | gaagctccct | 3450 |
| 3451 | cgtgcgctct | cctgttccga | ccctgccgct | taccggatac | ctgtccgcct | 3500 |
| 3501 | ttctcccttc | gggaagcgtg | gcgctttctc | atagctcacg | ctgtaggtat | 3550 |
| 3551 | ctcagttcgg | tgtaggtcgt | tcgctccaag | ctgggctgtg | tgcacgaacc | 3600 |
| 3601 | ccccgttcag | cccgaccgct | gcgccttatc | cggtaactat | cgtcttgagt | 3650 |
| 3651 | ccaacccggt | aagacacgac | ttatcgccac | tggcagcagc | cactggtaac | 3700 |
| 3701 | aggattagca | gagcgaggta | tgtaggcggt | gctacagagt | tcttgaagtg | 3750 |
| 3751 | gtggcctaac | tacggctaca | ctagaaggac | agtatttggt | atctgcgctc | 3800 |
| 3801 | tgctgaagcc | agttaccttc | ggaaaaagag | ttggtagctc | ttgatccggc | 3850 |
| 3851 | aaacaaacca | ccgctggtag | cggtggtttt | tttgtttgca | agcagcagat | 3900 |
| 3901 | tacgcgcaga | aaaaaaggat | ctcaagaaga | tcctttgatc | ttttctacgg | 3950 |
| 3951 | ggtctgacgc | tcagtggaac | gaaaactcac | gttaagggat | tttggtcatg | 4000 |
| 4001 | aacaataaaa | ctgtctgctt | acataaacag | taatacaagg | ggtgttatga | 4050 |
| 4051 | gccatattca | acgggaaacg | tcttgctcta | ggccgcgatt | aaattccaac | 4100 |
| 4101 | atggatgctg | atttatatgg | gtataaatgg | gctcgcgata | atgtcgggca | 4150 |
| 4151 | atcaggtgcg | acaatctatc | gattgtatgg | gaagcccgat | gcgccagagt | 4200 |
| 4201 | tgtttctgaa | acatggcaaa | ggtagcgttg | ccaatgatgt | tacagatgag | 4250 |
| 4251 | atggtcagac | taaactggct | gacggaattt | atgcctcttc | cgaccatcaa | 4300 |
| 4301 | gcattttatc | cgtactcctg | atgatgcatg | gttactcacc | actgcgatcc | 4350 |
| 4351 | ccgggaaaac | agcattccag | gtattagaag | aatatcctga | ttcaggtgaa | 4400 |
| 4401 | aatattgttg | atgcgctggc | agtgttcctg | cgccggttgc | attcgattcc | 4450 |
| 4451 | tgtttgtaat | tgtcctttta | acagcgatcg | cgtatttcgt | ctcgctcagg | 4500 |
| 4501 | cgcaatcacg | aatgaataac | ggtttggttg | atgcgagtga | ttttgatgac | 4550 |
| 4551 | gagcgtaatg | gctggcctgt | tgaacaagtc | tggaaagaaa | tgcataaact | 4600 |
| 4601 | tttgccattc | tcaccggatt | cagtcgtcac | tcatggtgat | ttctcacttg | 4650 |
| 4651 | ataaccttat | ttttgacgag | gggaaattaa | taggttgtat | tgatgttgga | 4700 |
| 4701 | cgagtcggaa | tcgcagaccg | ataccaggat | cttgccatcc | tatggaactg | 4750 |
| 4751 | cctcggtgag | ttttctcctt | cattacagaa | acggcttttt | caaaaatatg | 4800 |
| 4801 | gtattgataa | tcctgatatg | aataaattgc | agtttcattt | gatgctcgat | 4850 |
| 4851 | gagtttttct | aagaattaat | tcatgagcgg | atacatattt | gaatgtattt | 4900 |
| 4901 | agaaaaataa | acaaataggg | gttccgcgca | catttccccg | aaaagtgcca | 4950 |
| 4951 | cctgaaattg | taaacgttaa | tattttgtta | aaattcgcgt | taaatttttg | 5000 |
| 5001 | ttaaatcagc | tcatttttta | accaataggc | cgaaatcggc | aaaatccctt | 5050 |
| 5051 | ataaatcaaa | agaatagacc | gagatagggt | tgagtgttgt | tccagtttgg | 5100 |
| 5101 | aacaagagtc | cactattaaa | gaacgtggac | tccaacgtca | aagggcgaaa | 5150 |
| 5151 | aaccgtctat | cagggcgatg | gcccactacg | tgaaccatca | ccctaatcaa | 5200 |
| 5201 | gttttttggg | gtcgaggtgc | cgtaaagcac | taaatcggaa | ccctaaaggg | 5250 |
| 5251 | agcccccgat | ttagagcttg | acggggaaag | ccggcgaacg | tggcgagaaa | 5300 |
| 5301 | ggaagggaag | aaagcgaaag | gagcgggcgc | tagggcgctg | gcaagtgtag | 5350 |
| 5351 | cggtcacgct | gcgcgtaacc | accacacccg | ccgcgcttaa | tgcgccgcta | 5400 |
| 5401 | cagggcgcgt | cccattcgcc | a | 5421 |
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pET-30b(+)
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