pEX13

质粒编号:
质粒名称: pEX13
其他名称:
质粒大小（bp）: 5813
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
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蛋白标签:
测序引物:
GenBank号: X03174
其他属性: Created by Moore, July 1995, under contract with NCBI. This strain is resistant to 100 ug/ml ampicillin. (personal communication) This is one of three bacterial expression vectors (pEX11, ATCC 37708; pEX12, ATCC 37709; and pEX13, ATCC 37710) which differ by translational reading frames and which express cro-lacZ fusion proteins regulated by the PR promoter of bacteriophage lambda. [2] This was constructed from pEX3 by ligating a synthetic oligonucleotide into pEX3 cut with XmaI and BamHI. The oligonucleotide added several unique restriction sites. [1] The recombinant fusion protein generated using this vector is not solubilized in Triton X-100 lysis procedures and is thus easily purified, resistant to proteolysis, and easily detected by direct immunoscreening. [1] Restriction digests of the clone give the following sizes (kb): EcoRI–5.8; BamHI–5.8; PstI–5.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli POP2136 [ATCC 47049], E.coli. Related vectors: pEX3.
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