m7G(5′)ppp(5′)A帽结构类似物

3′‐Modified Oligonucleotides and their Conjugates

Sephadex G‐25 resin (Amersham Pharmacia Biotech) 1 M triethylammonium acetate (TEAA) 14% (v/v) aqueous

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

) N 2 ‐Isobutyryl‐5′‐O ‐(4,4′‐dimethoxytrityl)‐2′‐O ‐propargyl‐3′‐O ‐[(N,N ‐diisopropylamino) 2‐cyanoethyloxyphosphinyl]guanosine ( 3 , ChemGenes) 5.5 M tert ‐butyl

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

, and Klenow fragment exo(−) (Kf−). Primer was 5′‐end‐labeled using [γ‐32 P]ATP and polynucleotide kinase. Polymerization reactions were carried out with primer (3.5 µM), template (5 µM), all dNTPs and dNTPαS (0.1 mM each, final concentration), and Kf− (0.015 µ