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- 英文名：
EpiQuik Global Tri-Methyl Histone H3-K9 Quantification Kit (Colorimetric)
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- 通用Tri-Methyl组蛋白H3-K9定量试剂盒(Colorimetric) 详细信息：
-
Product Overview
The EpiQuik™ Global Tri-Methyl Histone H3-K9 Quantification Kit (Colorimetric) is a convenient package of tools that allows the experimenter to specifically measure global tri-methylation of histone H3-K9 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
WHY CHOOSE THE EPIQUIK™ GLOBAL TRI-METHYL HISTONE H3-K9 QUANTIFICATION KIT (COLORIMETRIC)?
- Quick and efficient, which can be finished within 2.5 hours.
- Innovative colorimetric assay with no need for radioactivity, electrophoresis, and chromatography.
- Specifically capture tri-methylated H3-K9 with the detection limit as low as 2 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
- The control is conveniently included for quantification of the amount of tri-methylated H3-K9.
- Strip microplate format makes the assay flexible: manual or high throughput.
- Simple, reliable, and consistent assay conditions.
通用Tri-Methyl组蛋白H3-K9定量试剂盒(Colorimetric) Principle & Procedure
The EpiQuik™ Global Tri-Methyl Histone H3-K9 Quantification Kit (Colorimetric) is designed for measuring global histone H3-tr-methylation. In an assay with this kit, the tri-methylated histone H3 at lysine 9 is captured to the strip wells coated with anti-tri-methyl H3-K9 antibody. The captured tri-methylated histone H3-K9 can be then detected with a labeled detection antibody followed by color development reagent. The ratio of tri-methylated H3-K9 is proportional to the intensity of absorbance. The absolute amount of tri-methylated H3-K9 can be quantitated by comparing to the standard control.
SCHEMATIC PROCEDURE: 通用Tri-Methyl组蛋白H3-K9定量试剂盒(Colorimetric) Product Components
C1 (10X wash buffer)
C2 (antibody buffer)
C3 (detection antibody, 1 mg/ml)*
C4 (color developer)
C5 (stop solution)
Standard control (100 µg/ml)*
8 well sample strips (with frame)
8 well standard control strips
User guide
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zkA055Hu 胰岛素样生长因子结合蛋白4(IGFBP4)ELISA试剂盒 检测范围
zkA056Hu 白介素10(IL10)ELISA试剂盒 检测范围
zkA057Hu 白介素11(IL11)ELISA试剂盒 检测范围
zkA058Hu 白介素12B(IL12B)ELISA试剂盒 检测范围
zkA059Hu 白介素12A(IL12A)ELISA试剂盒 检测范围
zkA061Hu 白介素15(IL15)ELISA试剂盒 检测范围
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zkA065Hu 白介素1受体Ⅱ(IL1R2)ELISA试剂盒 检测范围
zkA066Hu 白介素1受体Ⅰ(IL1R1)ELISA试剂盒 检测范围
zkA071Hu 白介素1α(IL1α)ELISA试剂盒 检测范围
zkA073Hu 白介素2(IL2)ELISA试剂盒 检测范围
zkA076Hu 白介素3(IL3)ELISA试剂盒 检测范围
zkA077Hu 白介素4(IL4)ELISA试剂盒 检测范围
* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.通用Tri-Methyl组蛋白H3-K9定量试剂盒(Colorimetric) User Guide & MSDS
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