- 询价
- 美国eBioscince
- ZK-0019543
- 美国/中国
- 2025年11月20日
企业认证
- 详细信息
- 技术资料
- 库存：
1
- 英文名：
Methylamp One-Step DNA Modification Kit
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- 一步法DNA甲基化修饰试剂盒详细信息：
-
Product Overview
The Methylamp™ One-Step DNA Modification Kit is a complete set of essential components which enables the experimenter to perform DNA methylation analysis using Epigenteks uniquely simplified and streamlined bisulfite method. The entire procedure can be completed within a mere 1 hour and 45 minutes and produces far superior results than any competitor kits. The Methylamp™ One-Step DNA Modification Kit is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
WHY CHOOSE THE METHYLAMP™ ONE-STEP DNA MODIFICATION KIT?- The number one fastest procedure available. The entire experiment can be finished within 1 hour and 45 minutes.
- Completely converts unmethylated cytosine into uracil: modified DNA > 99.98%.
- The lowest degradation of DNA in the modification process: more than 90% of DNA loss can be prevented.
- The lowest requirement of starting DNA for modification: only 50 pg or 20 cells.
- Extremely simple, reliable, and consistent modification conditions.
一步法DNA甲基化修饰试剂盒Principle & Procedure
The Methylamp™ One-Step DNA Modification Kit contains all reagents required for bisulfite conversion on a DNA sample. DNA is denatured by heating, which allows DNA denaturation and bisulfite modification to be carried out simultaneously. In the modification process, bisulfite reagent reacts specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enable DNA tightly to bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA then can be eluted and stably stored at –20°C for up to 2 months.
SCHEMATIC PROCEDURE: COMPARATIVE OVERVIEW: 一步法DNA甲基化修饰试剂盒Product Components
G1 (DNA modification powder)
G2 (DNA modification)
G3 (balance solution)
G4 (modified DNA capture)
G5 (modified DNA cleaning)
G6 (modified DNA elution)
F-spin column
F-collection tube
User guide
zk412-01 蛋白复性液
zk412-02 蛋白复性液
zk301 蛋白标准(5mg/ml BSA)
zk302
zk303 PMSF(100mM)
zk304 5×Tris-甘氨酸电泳缓冲液
zk305-1 SDS-PAGE电泳液(10X)
zk306 SDS-PAGE蛋白上样缓冲液(5X)
zk307 非变性PAGE蛋白上样缓冲液(5X)
zk308
zk309 10%过 硫 酸 铵
zk310 30% 丙 烯 酰 胺 (29:1)
zk311 40% 丙 烯 酰 胺 (39:1)
zk312-1 0.5M DTT
zk312-2 0.5M DTT
zk313 10% SDS
zk314 TEMED
zk315 1M Tris-HCl,pH6.8
zk316 1.5M Tris-HCl,pH8.8
zk401 Western转膜缓冲液 （20x）
zk402 Western一/二抗稀释液
zk403 Western封闭液(BSA型）
zk404 Western洗涤液
zk405 Western一抗二抗去除液
zk406 BCIP工作液 50mg/ml
zk407 NBT工作液 50mg/ml
zk408 10% Sodium Azide (叠 氮 钠)
zk101-1 人 IgG
zk101-2 人 IgG
zk101-3 人 IgG
zk101-4 人 IgG
zk102-1 人 IgG ( 液体-Ph 7.2 PBS )
zk102-2 人 IgG ( 液体-Ph 7.2PBS )
zk102-3 人 IgG ( 液体-Ph 7.2 PBS )
zk103-1 兔 IgG
zk103-2 兔 IgG一步法DNA甲基化修饰试剂盒User Guide & MSDS
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