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- 详细信息
- 技术资料
- 库存:
大量
- 英文名:
pCYM02
- 保质期:
1年
- 供应商:
再康生物
- 保存条件:
低温运输,-20℃保存
质粒编号:
质粒名称: pCYM02
其他名称:
质粒大小(bp): 8510
抗性:
质粒类型: Unspecified
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
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蛋白标签:
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GenBank号:
其他属性: Expresses beta-galactosidase activity in E.coli, S. cerevisiae, and mouse cells, and thus can be used as a control to calibrate transformation efficency. Constructed by inserting a 3.048 kb EcoRI/DraI fragment from pMC1403 into pCYM2 cut with BamHI (nt 427), linkered to add an EcoRI site, and cut with BalI (nt 702). Restriction digests of the clone give the following sizes (kb): BamHI–8.4; BglII/EcoRI–5.0, 3.5; EcoRI–8.4. (ATCC staff) Shuttle expression vector which includes a reporter group. (ATCC staff) The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter. Also permits blue/white detection in E.coli. Medium is 1227 LB plus ampicillin. Hosts: E.coli CM5alpha, Saccharomyces cerevisiae, vertebrate cells, E.coli, Saccharomyces cerevisiae W303-1B/D, mouse C11D cell line.
质粒图谱:
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