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- 2025年11月20日
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- 详细信息
- 技术资料
- 库存：
1
- 英文名：
EpiQuik Methyl-Histone H3-K9 ChIP Kit
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- 甲基组蛋白H3-K9免疫沉淀ChIP试剂盒详细信息：
-
Product Overview
The EpiQuik™ Methyl-Histone H3-K9 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3-K9 methylation and DNA in vivo efficiently. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Methyl-Histone H3-K9 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray.
WHY CHOOSE THE EPIQUIK™ METHYL-HISTONE H3-K9 ChIP KIT?- The fastest procedure available, which can be finished within 5 hours.
- Strip microplate format makes the assay flexible: manual or high throughput.
- Columns for DNA purification are included: save time and reduce labor.
- Compatible with all DNA amplification-based approaches.
- Simple, reliable, and consistent assay conditions.
甲基组蛋白H3-K9免疫沉淀ChIP试剂盒Principle & Procedure
The EpiQuik™ Methyl-Histone H3-K9 ChIP Kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for methyl-histone H3-K9 from mammalian cells. Particularly, this kit includes a ChIP-grade methyl-histone H3-K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured methyl-histone H3-K9 protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.
SCHEMATIC PROCEDURE: 甲基组蛋白H3-K9免疫沉淀ChIP试剂盒Product Components
CP1 (wash buffer)
CP2 (antibody buffer)
CP3A (pre-lysis buffer)
CP3B (lysis buffer)
CP4 (CHIP dilution buffer)
CP5 (DNA release buffer)
CP6 (reverse buffer)
CP7 (binding buffer)
CP8 (elution buffer)
Protease inhibitor cocktail (100x)*
Proteinase K (10 mg/ml)*
Normal mouse IgG (0.5 mg/ml)*
Anti-dimethyl-H3-K9 (1 mg/ml)*
8-well assay strips (with frame)
8-well strip caps
F-spin column
F-collection tube
User guide
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* Spin the solution down to the bottom before use.甲基组蛋白H3-K9免疫沉淀ChIP试剂盒User Guide & MSDS
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