pADbeta

质粒编号:
质粒名称: pADbeta
其他名称:
质粒大小（bp）: 7069
抗性: ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
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其他属性: ATCC size is 7200 bp. The order of the major features in this plasmid is: pUC19 – EcoRI – adenovirus 2 major late promoter/tripartite leader/splice signal – NotI – lacZ – NotI – SV40 late polyadenylation signal – XbaI – HindIII – pUC19. [1] The lacZ gene may be excised by NotI and replaced with alternative sequences. [1] Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining. [1] Designed primarily as an enhancer trap vector. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. [1] Restriction digests of the clone give the following sizes (kb): EcoRI–7.2; HindIII–3.9, 3.3; EcoRI/HindIII–3.9, 2.7, 0.6; NotI–3.6 (doublet); BamHI–7.0, 0.2. (ATCC staff) Medium is -1 TB plus ampicillin (200 ug/ml). Hosts: E.coli DH5alpha, E.coli, monkey COS cells, vertebrate cells. Related vectors: pUC19, pNASSbeta, pCMVbeta, pSVbeta, pTKbeta.
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