- 询价
- R&D
- ZK-0102221
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
1 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- Cytokeratin 19 (RCK 108) Monoclonal Antibody详细信息：
-
Description Monoclonal Antibody against Cytokeratin 19, clone RCK 108 Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone RCK 108 Form Cell Culture Supernatant (dialysed against 0.05M Tris/HCl, pH 7.2 + 0.015M sodium azide) Host Mouse Species Reactivity Human IsoType Cytokeratin 19 (RCK 108) Monoclonal Antibody IgG1 Specificity Cytokeratin 19 (CK 19) belongs to the intermediate filaments, which create a cytoskeleton in almost all cells. In contrast to other intermediate filaments, cytokeratins (CKs) are made up of a highly complex multigene family of polypeptides with molecular masses ranging from 40 to 68 kDa. Twenty distinct CK polypeptides have been revealed in various human epithelial cells. They can be divided into an acidic (type I) and a neutral-basic (type II) subfamily. CK 19 belongs to the acidic type of cytokeratins, and is a low molecular mass cytokeratin (40 kDa) typically expressed in simple epithelia, superficial, intermediate and basal cells of transitional epithelium as well as luminal, basal and myoepithelial cells in a few complex epithelia. CK 19 is normally not expressed in stratified squamous epithelia, but may be present in modified squamous epithelium invaded by lymphocytes as well as in basal cells in non-keratinizing stratified squamous epithelium.
In Western blotting of cytoskeleton preparations from the T24 and RT4 cell lines as well as the human squamous carcinoma cell line HaCaT, the antibody labels a single band of 40 kDa corresponding to CK 19.
In immunocytochemistry on different human tissues, the antibody labels only epithelia, whereas cells known not to contain CK 19, e.g. hepatocytes, are not labeled.
Cells labelled by the antibody display a cytoplasmic staining pattern.
This antibody was developed against a cytoskeletal preparation from human bladder cancer cell line T24.Uses This antibody is effective in immunoblotting, immunocytochemistry (ICC) and immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - Western blot: 1:50 - 1:100*
- IHC: 1:50 - 1:100**
Paraffin sections: The antibody can be used for labelling formalin-fixed, paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with proteinase K or heat-induced epitope retrieval is required. The tissue sections should not dry out during the treatment or during the following immunocytochemical staining procedure.
Frozen sections and cell preparations: The antibody can be used for labelling frozen sections.Notes *Predicted MW = 40kDa
**Using 5 minutes proteolytic epitope retrieval and 30 minutes incubation at room temperature with the primary antibodyStorage Cytokeratin 19 (RCK 108) Monoclonal Antibody Store between 2-8°C. References Moll R. Cytokeratins as markers of differentiation in the diagnosis of epithelial tumors. Subcell Biochem 31:205-62, 1998.
Kwaspen FHL, Smedts FMM, Broos A, Bulten H, Debie WMH et al. Reproducible and highly sensitive detection of the broad spectrum epithelial marker keratin 19 in routine cancer diagnosis. Histopathol 31:503-16, 1997.
Dalal P, Shousha S. keratin 19 in paraffin sections of medullary carcinoma and other benign and malignant breast lesions. Mod Pathol 8:413-6, 1995.
Moll R, Löwe A, Laufer J, Franke WW. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol 140:427-47, 1992.
Smedts F, Ramaekers F, Troyanovsky S, Pruszczynski M, Link M, et al. Keratin expression in cervical cancer. Am J Pathol 141:497-511, 1992.
Lane EB, Alexander CM. Use of keratin antibodies in tumor diagnosis. In: Osborn M, ed. Seminars in Cancer Biology. Antibodies in Diagnosis and Therapy. Philadelphia: Saunders, 1990:165-79.
Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11-24, 1982.Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Cytokeratin 19 (RCK 108) Monoclonal Antibody
- zkB074Hu 多聚免疫球蛋白受体(PIGR)ELISA试剂盒 检测范围
zkB075Hu 促胰液素(SCT)ELISA试剂盒 检测范围
zkB077Hu ⅡD组磷脂酶A2(PLA2G2D)ELISA试剂盒 检测范围
zkB080Hu S100钙结合蛋白A12(S100A12)ELISA试剂盒 检测范围
zkB081Hu 70kDa热休克蛋白1A(HSPA1A)ELISA试剂盒 检测范围
zkB084Hu 甘丙肽(GAL)ELISA试剂盒 检测范围
zkB083Hu 线粒体超氧化物歧化酶(SOD2)ELISA试剂盒 检测范围
zkB085Hu 肠碱性磷酸酶(ALPI)ELISA试剂盒 检测范围
zkB089Hu 谷胱甘肽S转移酶κ1(GSTκ1)ELISA试剂盒 检测范围
zkB090Hu 谷胱甘肽S转移酶π1(GSTπ1)ELISA试剂盒 检测范围
zkB091Hu 肝肾骨碱性磷酸酶(ALPL)ELISA试剂盒 检测范围
zkB092Hu 骨涎蛋白(BSP)ELISA试剂盒 检测范围
zkB094Hu 凝血因子ⅩⅢA1肽(F13A1)ELISA试剂盒 检测范围
zkB098Ge 花生四烯酸(AA)ELISA试剂盒 检测范围
zkB107Hu 低密度脂蛋白(LDL)ELISA试剂盒 检测范围
zkB109Hu 肌细胞生成素(MYOG)ELISA试剂盒 检测范围
zkA746Hu 成纤维细胞生长因子23(FGF23)ELISA试剂盒 检测范围
zkA747Hu 补体1q(C1q)ELISA试剂盒 检测范围
zkA675Hu 凝血因子Ⅴ(F5)ELISA试剂盒 检测范围
zkA676Hu 凝血因子ⅩⅢ(F13)ELISA试剂盒 检测范围
zkA677Hu 凝血因子Ⅻ(F12)ELISA试剂盒 检测范围
zkA748Hu 三叶因子2(TFF2)ELISA试剂盒 检测范围
zkB622Hu 表面活性物质关联蛋白B(SPB)ELISA试剂盒 检测范围
zkB623Hu 表面活性物质关联蛋白C(SPC)ELISA试剂盒 检测范围
zkA656Hu 二胺氧化酶(DAO)ELISA试剂盒 检测范围
zkB572Hu 淋巴细胞功能关联抗原1α(LFA1α)ELISA试剂盒 检测范围
zkB705Hu 防御素α1(DEFα1)ELISA试剂盒 检测范围
zkB706Hu 脂质运载蛋白1(LCN1)ELISA试剂盒 检测范围
zkA753Hu Toll样受体4(TLR4)ELISA试剂盒 检测范围
zkB955Hu 白介素17F(IL17F)ELISA试剂盒 检测范围
zkC019Hu 血小板激活因子乙酰水解酶2(PAFAH2)ELISA试剂盒 检测范围
zkB818Mu 血管内皮生长因子受体1(VEGFR1)ELISA试剂盒 检测范围
zkC026Hu Salusinβ蛋白(Salusinβ)ELISA试剂盒 检测范围
zkC027Hu O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)ELISA试剂盒 检测范围
zkC028Hu 白介素28B(IL28B)ELISA试剂盒 检测范围
zkC029Hu 白介素29(IL29)ELISA试剂盒 检测范围
zkC031Hu 白介素4受体(IL4R)ELISA试剂盒 检测范围
zkB828Hu 载脂蛋白C4(APOC4)ELISA试剂盒 检测范围
zkA685Hu CD14分子(CD14)ELISA试剂盒 检测范围
zkB117Hu 甲胎蛋白异质体3(αFPL3)ELISA试剂盒 检测范围
zkB119Hu 金属硫蛋白1(MT1)ELISA试剂盒 检测范围
zkB120Hu 精氨酸酶(Arg)ELISA试剂盒 检测范围
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
Cytokeratin 19 (RCK 108) Monoclonal Antibody
询价
