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- 详细信息
- 文献和实验
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- 靶点:
来电咨询
- 克隆性:
多克隆
- 标记物:
见 说 明 书
- 宿主:
Goat,Rabbit,Mouse
- 适应物种:
人,大鼠,小鼠,兔
- 形态:
液态/粉状
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 保存条件:
-20℃保存
- Nonmuscle Myosin Heavy Chain II-B Polyclonal Antibody, Purified详细信息:
-
Description Polyclonal Antibody against Nonmuscle Myosin II Heavy Chain Isoform B Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Form Affinity Purified Antibody (Stored in TBS + 0.03% Thimerosal) Host Rabbit Species Reactivity Human, Rodent [Ab] Nonmuscle Myosin Heavy Chain II-B Polyclonal Antibody, Purified 1 mg/mL Specificity This antibody was raised against the peptide SDVNETQPPQSE corresponding to the C-terminus of nonmuscle myosin heavy chain isoform B (MHC-B). Uses This antibody is effective in immunoblotting (WB) and immunofluorescence (IF). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - WB: 1:1,000*
- IF: 1:500
Notes *Predicted MW = 200 kDa Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. References Ma X, Bao J, Adelstein RS. Loss of cell adhesion causes hydrocephalus in nonmuscle myosin II-B-ablated and mutated mice. Mol Biol Cell 18(6):2305-12, 2007. [IF]
Golomb E, Ma X, Jana SS, Preston YA, Kawamoto S, Shoham NG, Goldin E, Conti MA, Sellers JR, Adelstein RS. Identification and characterization of nonmuscle myosin II-C, a new member of the myosin II family. J Biol Chem 279(4):2800-8, 2004. [WB, IF]
Nonmuscle Myosin Heavy Chain II-B Polyclonal Antibody, Purified Song J, Goetz BD, Baas PW, Duncan ID. Cytoskeletal reorganization during the formation of oligodendrocyte processes and branches. Mol Cell Neurosci 17(4):624-636, 2001.
Kelley CA, Sellers JR, Gard DL, Bui D, Adelstein RS, Baines IC. Xenopus nonmuscle myosin heavy chain isoforms have different subcellular localizations and enzymatic activities. J Cell Biol 134:675-687, 1996.
Kelley CA, Oberman F, Yisraeli JK, Adelstein RS. A Xenopus nonmuscle myosin heavy chain is phosphorylated by cyclin-p34cdc 2 kinase during meiosis. J Biol Chem 270:1395-1401, 1995.
Phillips CL, Yamakawa K, Adelstein RS. Cloning of the cDNA encoding human nonmuscle myosin heavy chain-B and analysis of human tissues with isoform-specific antibodies. J Muscl Res Cell Motil 16:379-389, 1995.Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Nonmuscle Myosin Heavy Chain II-B Polyclonal Antibody, Purified
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