pDO184

质粒编号:
质粒名称: pDO184
其他名称:
质粒大小（bp）: 9820
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: U03460
其他属性: Restriction digests of the clone give the following sizes (kb): EcoRI–10.0; HindIII–10.0; XbaI–9.2, 1.0; BglI–2.8, 2.35 (doublet), 1.55, 0.75, 0.28; PstI–4.0, 3.7, 2.5. (ATCC staff) Plasmid contains the following restriction sites (approximate kb from nt 1): BamHI–4.42, 4.77; BglI–1.96, 4.60, 5.32, 5.56, 7.88, 9.42; EcoRI–4.40; HindIII–4.45; PstI–0.65, 4.44, 8.00; PvuI–0.97, 4.57, 8.13; SmaI–4.41; XbaI–3.45, 4.42. (GenBank/EMBL/DDBJ) A series of overlapping deletion segments can be generated by partial digestion with a restriction enzyme not found in the vector, followed by complete digestion with lambda terminase. The following restriction sites do not cut pDO17: Acc65I AflII ApaI AscI AvrII Ecl136II KpnI NotI PacI PmeI PmlI SacI SfiI SnaBI SpeI SrfI Sse8387I SwaI XbaI. [1] Cosmid vector containing two compatible origins of replication, for generating bidirectional deletions of large inserts to facilitate sequencing. Resulting fragments are blunted and circularized by ligation, and transformants are selected for either ampicillin or kanamycin resistance. AmpR transformants can be sequenced in the clockwise direction using the cosL primer 5'-TCATAAATAGCGAAAACC-3'. KanR transformants can be sequenced in the counterclockwise direction using the cosR primer 5'-ACTTTACGGGTCCTTTCCG-3'. [2] Growth: 1065 plus ampicillin (50 ug/ml) plus kanamycin (20 ug/ml) 30C Deposited by: Ahmed A.L. NCBI gi: 424108 Hosts: E.coli, E.coli AA102. Related vectors: pBR322, pDO2, pDO17, pDO6, pDO19.
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