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- 详细信息
- 文献和实验
- 技术资料
- 库存：
60
- 英文名：
ThrombopoietinHuman Recombinant, His Tag
- 保质期：
12个月
- 供应商：
上海再康生物科技有限公司
- 保存条件：
见 说 明 书
- 规格：
2μg 10μg 1mg
- TPO Human, His
- ThrombopoietinHuman Recombinant, His Tag
CATALOGUE NUMBER
CYT-890
SYNONYMS
MPL Ligand, THCYT1, MPLLG, TPO, ML, Thrombopoietin Nirs Variant 1, MKCSF, Thrombopoietin, Myeloproliferative Leukemia Virus Oncogene Ligand, Megakaryocyte Growth and Development Factor, Megakaryocyte Colony-Stimulating Factor, C-Mpl Ligand, MGDF, Megakaryocyte Stimulating Factor, Prepro-Thrombopoietin.INTRODUCTION
| Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney which regulates the production of platelets by the bone marrow. TPO stimulates the production as well as differentiation of megakaryocytes, the bone marrow cells which fragment into large numbers of platelets. |
DESCRIPTION
TPO produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 340 amino acids (22-353aa.) and having a molecular mass of 36.5kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57KDa).TPO is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques..
SOURCE
Sf9, Insect cell.PHYSICAL APPEARANCE
Sterile Filtered clear solution.FORMULATION
TPO protein solution (0.25mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol.STABILITY
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
PURITY
Greater than 85% as determined by SDS-PAGE.AMINO ACID SEQUENCE
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSGLLETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH ELLNGTRGLF PGPSRRTLGA PDISSGTSDT GSLPPNLQPG YSPSPTHPPT GQYTLFPLPP TLPTPVVQLH PLLPDPSAPT PTPTSPLLNT SYTHSQNLSQ EGLEHHHHHH.
USAGE
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文献和实验28 Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use
This chapter will describe a method used to produce human megakaryocytes in vitro (see Fig. 1 ), in high yield, for a clinical product to supplement progenitor cell transplants. Producing significant numbers of megakaryocytes from human
Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood
This chapter describes a robust method for the generation of iPS cells from non-cultured human cord blood cells. We describe the preparation of the CD34+ fraction from cord blood mononuclear cells, the protocols to determine the pluripotency
approach yielded specific ligands for human thrombopoietin receptor (TPO-R). Thrombopoietin (TPO) is the primary regulator of platelet production and acts by dimerization of its cognate receptor. Chemical cross linking of two anti TPO-R Microbodies™
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