- 询价
- PEPROTECH
- ZK-0097819
- 中国/美国
- 2025年11月20日
2 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 英文名：
Recombinant Tenascin R (TNR)
- 供应商：
上海再康
- 保存条件：
-20℃
肌腱蛋白R(TNR)重组蛋白
Recombinant Tenascin R (TNR)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | RPB328Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 90% |
| UOM | 50ug |
| Predicted Molecular Mass | 58.2kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Pro329~Gln588 with two N-terminal Tags, His-tag and GST-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
肌腱蛋白R(TNR)重组蛋白 SEQUENCES
The sequence of the target protein is listed below.
PE DLRVAGISDR SIELEWDGPM AVTEYVISYQ PSLGGLQLQQ RVPGDWSGVT ITELEPGLTY NISVYAVISN ILSLPITAKV ATHLSTPQGL QFKTITETTV EVQWEPFSFS FDGWEISFTP KNNEGGVIAQ LPSDVTSFNQ TGLKPGEEYI VNVVALKEQA RGPPTSASVS TVIDGPTQIL VRDVSDTVAF VEWTPPRAKV DFILLKYGLV GGEGGKTTFR LQPPLSQYSV QALRPGSRYE VSISAVRGTN ESDASSTQ
PE DLRVAGISDR SIELEWDGPM AVTEYVISYQ PSLGGLQLQQ RVPGDWSGVT ITELEPGLTY NISVYAVISN ILSLPITAKV ATHLSTPQGL QFKTITETTV EVQWEPFSFS FDGWEISFTP KNNEGGVIAQ LPSDVTSFNQ TGLKPGEEYI VNVVALKEQA RGPPTSASVS TVIDGPTQIL VRDVSDTVAF VEWTPPRAKV DFILLKYGLV GGEGGKTTFR LQPPLSQYSV QALRPGSRYE VSISAVRGTN ESDASSTQ
肌腱蛋白R(TNR)重组蛋白 USAGE
Reconstitute in sterile ddH2O.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
肌腱蛋白R(TNR)重组蛋白 About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
,Geoffery Bien,Susan Yao,Yong Zhu,Gary Siuzdak,Hans R. Scohler,Lingxun Duan,Sheng Ding
摘要 基因重组蛋白在大肠杆菌中表达时，由于表达量高，往往形成无生物活性的包涵体。包涵体必须经过变性和复性的过程才能获得有活性的重组蛋白。如何提高基因重组蛋白质的复性率，是生物工程技术的一个研究热点。对近年来的重组蛋白质的复性方法做一评述，为研究蛋白质折叠以及复性技术的进一步应用提供依据。 关键词 重组蛋白 包涵体 复性 二硫键 到目前为止，人们表达的重组蛋白质已有4000多种，其中用E.coli表达的蛋白质要占90%以上，尽管基因重组技术为大规模生产目标蛋白质提供
杆菌的包涵体可采用离心收获。 洗涤 包涵体常用的洗涤试剂为中性去垢剂和还原剂。包涵体中主要由重组蛋白组成，同时也含有一些外膜蛋白（Omp C、Omp F、Omp A）、16S 和 23S、5S的r RNA、质粒 DNA 和其它杂质。去除这些杂质，可采用去垢剂 Triton X-100、脱氧胆酸盐和低浓度的变性剂等对包涵体充分洗涤。包涵体洗涤的洁净程度，对于后续的纯化步骤十分重要，包涵体纯度高，可以大大降低后续的纯化压力。但是，变性剂尿素的使用应该注意的是大肠杆菌外膜蛋白Omp T（37 kD
技术资料暂无技术资料 索取技术资料
肌腱蛋白R(TNR)重组蛋白
询价
