pCN40

Constructed by replacing the PvuII/PstI fragment of pCN39 (ATCC 77096) with a 1.9 kb PvuII/PstI fragment of pKT231 encoding streptomycin resistance. Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 & 1500), with the site at nt 536 between the repA promoter and coding sequences. (personal communication) The kanR gene can be insertionally inactivated by HindIII digestion. General purpose vector. (ATCC staff) Restriction digests of the clone give the following sizes (kb): HindIII–6.0; PstI/PvuII–4.1, 1.9; KpnI/SmaI–4.9, 1.1; SmaI–6.0. (ATCC staff) The order of the major features in this plasmid is:kanR – PvuII – strR – PstI – SmaI – BamHI – kanR – HindIII. Medium is 1236 LB plus kanamycin. Hosts: Pseudomonas aeruginosa PAO1024 [ATCC 47053], Pseudomonas, P.aeruginosa PAO1162, P.putida KT2440 [ATCC 47054].