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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pBR329
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号:
质粒名称: pBR329
其他名称:
质粒大小（bp）: 4150
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: L08859
其他属性: Revised 02-APR-1986 by F. Pfeiffer Reference [1] only sequenced bases 160-340 and 3440-3560. The other regions are from previously published sequences for pBR327 and Tn981, as listed in figure 5 of [1]. This plasmid does not carry the inverted duplication region found in pBR328. Medium is 1227 LB plus ampicillin. Hosts: E.coli RR1, E.coli. Related vectors: pBR328, Tn981, pBR325.
质粒图谱:
质粒序列:
| 1 | ttccggatga | gcattcatca | ggcgggcaag | aatgtgaata | aaggccggat | 50 |
| 51 | aaaacttgtg | cttatttttc | tttacggtct | ttaaaaaggc | cgtaatatcc | 100 |
| 101 | agctgaacgg | tctggttata | ggtacattga | gcaactgact | gaaatgcctc | 150 |
| 151 | aaaatgttct | ttacgatgcc | attgggatat | atcaacggtg | gtatatccag | 200 |
| 201 | tgattttttt | ctccatttta | gcttccttag | ctcctgatgt | ttgacagctt | 250 |
| 251 | atcatcgata | agctttaatg | cggtagttta | tcacagttaa | attgctaacg | 300 |
| 301 | cagtcaggca | ccgtgtatga | aatctaacaa | tgcgctcatc | gtcatcctcg | 350 |
| 351 | gcaccgtcac | cctggatgct | gtaggcatag | gcttggttat | gccggtactg | 400 |
| 401 | ccgggcctct | tgcgggatat | cgtccattcc | gacagcatcg | ccagtcacta | 450 |
| 451 | tggcgtgctg | ctagcgctat | atgcgttgat | gcaatttcta | tgcgcacccg | 500 |
| 501 | ttctcggagc | actgtccgac | cgctttggcc | gccgcccagt | cctgctcgct | 550 |
| 551 | tcgctacttg | gagccactat | cgactacgcg | atcatggcga | ccacacccgt | 600 |
| 601 | cctgtggatc | ctctacgccg | gacgcatcgt | ggccggcatc | accggcgcca | 650 |
| 651 | caggtgcggt | tgctggcgcc | tatatcgccg | acatcaccga | tggggaagat | 700 |
| 701 | cgggctcgcc | acttcgggct | catgagcgct | tgtttcggcg | tgggtatggt | 750 |
| 751 | ggcaggcccg | tggccggggg | actgttgggc | gccatctcct | tgcatgcacc | 800 |
| 801 | attccttgcg | gcggcggtgc | tcaacggcct | caacctacta | ctgggctgct | 850 |
| 851 | tcctaatgca | ggagtcgcat | aagggagagc | gtcgaccgat | gcccttgaga | 900 |
| 901 | gccttcaacc | cagtcagctc | cttccggtgg | gcgcggggca | tgactatcgt | 950 |
| 951 | cgccgcactt | atgactgtct | tctttatcat | gcaactcgta | ggacaggtgc | 1000 |
| 1001 | cggcagcgct | ctgggtcatt | ttcggcgagg | accgctttcg | ctggagcgcg | 1050 |
| 1051 | acgatgatcg | gcctgtcgct | tgcggtattc | ggaatcttgc | acgccctcgc | 1100 |
| 1101 | tcaagccttc | gtcactggtc | ccgccaccaa | acgtttcggc | gagaagcagg | 1150 |
| 1151 | ccattatcgc | cggcatggcg | gccgacgcgc | tgggctacgt | cttgctggcg | 1200 |
| 1201 | ttcgcgacgc | gaggctggat | ggccttcccc | attatgattc | ttctcgcttc | 1250 |
| 1251 | cggcggcatc | gggatgcccg | cgttgcaggc | catgctgtcc | aggcaggtag | 1300 |
| 1301 | atgacgacca | tcagggacag | cttcaaggat | cgctcgcggc | tcttaccagc | 1350 |
| 1351 | ctaacttcga | tcactggacc | gctgatcgtc | acggcgattt | atgccgcctc | 1400 |
| 1401 | ggcgagcaca | tggaacgggt | tggcatggat | tgtaggcgcc | gccctatacc | 1450 |
| 1451 | ttgtctgcct | ccccgcgttg | cgtcgcggtg | catggagccg | ggccacctcc | 1500 |
| 1501 | acctgaatgg | aagccggcgg | cacctcgcta | acggattcac | cactccaaga | 1550 |
| 1551 | attggagcca | atcaattctt | gcggagaact | gtgaatgcgc | aaaccaaccc | 1600 |
| 1601 | ttggcagaac | atatccatcg | cgtccgccat | ctccagcagc | cgcacgcggc | 1650 |
| 1651 | gcatctcggg | ccgcgttgct | ggcgtttttc | cataggctcc | gcccccctga | 1700 |
| 1701 | cgagcatcac | aaaaatcgac | gctcaagtca | gaggtggcga | aacccgacag | 1750 |
| 1751 | gactataaag | ataccaggcg | tttccccctg | gaagctccct | cgtgcgctct | 1800 |
| 1801 | cctgttccga | ccctgccgct | taccggatac | ctgtccgcct | ttctcccttc | 1850 |
| 1851 | gggaagcgtg | gcgctttctc | aatgctcacg | ctgtaggtat | ctcagttcgg | 1900 |
| 1901 | tgtaggtcgt | tcgctccaag | ctgggctgtg | tgcacgaacc | ccccgttcag | 1950 |
| 1951 | cccgaccgct | gcgccttatc | cggtaactat | cgtcttgagt | ccaacccggt | 2000 |
| 2001 | aagacacgac | ttatcgccac | tggcagcagc | cactggtaac | aggattagca | 2050 |
| 2051 | gagcgaggta | tgtaggcggt | gctacagagt | tcttgaagtg | gtggcctaac | 2100 |
| 2101 | tacggctaca | ctagaaggac | agtatttggt | atctgcgctc | tgctgaagcc | 2150 |
| 2151 | agttaccttc | ggaaaaagag | ttggtagctc | ttgatccggc | aaacaaacca | 2200 |
| 2201 | ccgctggtag | cggtggtttt | tttgtttgca | agcagcagat | tacgcgcaga | 2250 |
| 2251 | aaaaaaggat | ctcaagaaga | tcctttgatc | ttttctacgg | ggtctgacgc | 2300 |
| 2301 | tcagtggaac | gaaaactcac | gttaagggat | tttggtcatg | agattatcaa | 2350 |
| 2351 | aaaggatctt | cacctagatc | cttttaaatt | aaaaatgaag | ttttaaatca | 2400 |
| 2401 | atctaaagta | tatatgagta | aacttggtct | gacagttacc | aatgcttaat | 2450 |
| 2451 | cagtgaggca | cctatctcag | cgatctgtct | atttcgttca | tccatagttg | 2500 |
| 2501 | cctgactccc | cgtcgtgtag | ataactacga | tacgggaggg | cttaccatct | 2550 |
| 2551 | ggccccagtg | ctgcaatgat | accgcgagac | ccacgctcac | cggctccaga | 2600 |
| 2601 | tttatcagca | ataaaccagc | cagccggaag | ggccgagcgc | agaagtggtc | 2650 |
| 2651 | ctgcaacttt | atccgcctcc | atccagtcta | ttaattgttg | ccgggaagct | 2700 |
| 2701 | agagtaagta | gttcgccagt | taatagtttg | cgcaacgttg | ttgccattgc | 2750 |
| 2751 | tgcaggcatc | gtggtgtcac | gctcgtcgtt | tggtatggct | tcattcagct | 2800 |
| 2801 | ccggttccca | acgatcaagg | cgagttacat | gatcccccat | gttgtgcaaa | 2850 |
| 2851 | aaagcggtta | gctccttcgg | tcctccgatc | gttgtcagaa | gtaagttggc | 2900 |
| 2901 | cgcagtgtta | tcactcatgg | ttatggcagc | actgcataat | tctcttactg | 2950 |
| 2951 | tcatgccatc | cgtaagatgc | ttttctgtga | ctggtgagta | ctcaaccaag | 3000 |
| 3001 | tcattctgag | aatagtgtat | gcggcgaccg | agttgctctt | gcccggcgtc | 3050 |
| 3051 | aacacgggat | aataccgcgc | cacatagcag | aactttaaaa | gtgctcatca | 3100 |
| 3101 | ttggaaaacg | ttcttcgggg | cgaaaactct | caaggatctt | accgctgttg | 3150 |
| 3151 | agatccagtt | cgatgtaacc | cactcgtgca | cccaactgat | cttcagcatc | 3200 |
| 3201 | ttttactttc | accagcgttt | ctgggtgagc | aaaaacagga | aggcaaaatg | 3250 |
| 3251 | ccgcaaaaaa | gggaataagg | gcgacacgga | aatgttgaat | actcatactc | 3300 |
| 3301 | ttcctttttc | aatattattt | aagcatttat | cagggttatt | gtctcatgag | 3350 |
| 3351 | cggatacata | tttgaatgta | tttagaaaaa | taaacaaata | ggggttccgc | 3400 |
| 3401 | gcacatttcc | ccgaaaagtg | ccacctgacg | tctaagaaac | cattattatc | 3450 |
| 3451 | atgacattaa | cctataaaaa | taggcgtatc | acgaggccct | ttcgtctagg | 3500 |
| 3501 | catttgagaa | gcacacggtc | acactgcttc | cggtagtcaa | taaaccggta | 3550 |
| 3551 | aaccagcaat | agacataagc | ggctatttaa | cgaccctgcc | ctgaaccgac | 3600 |
| 3601 | gaccgggtcg | aatttgcttt | cgaatttctg | ccattcatcc | gcttattatc | 3650 |
| 3651 | acttattcag | gcgtagcacc | aggcgtttaa | gggcaccaat | aactgcctta | 3700 |
| 3701 | aaaaaattac | gccccgccct | gccactcatc | gcagtactgt | tgtaattcat | 3750 |
| 3751 | taagcattct | gccgacatgg | aagccatcac | aaacggcatg | atgaacctga | 3800 |
| 3801 | atcgccagcg | gcatcagcac | cttgtcgcct | tgcgtataat | atttgcccat | 3850 |
| 3851 | ggtgaaaacg | ggggcgaaga | agttgtccat | attggccacg | tttaaatcaa | 3900 |
| 3901 | aactggtgaa | actcacccag | ggattggctg | agacgaaaaa | catattctca | 3950 |
| 3951 | ataaaccctt | tagggaaata | ggccaggttt | tcaccgtaac | acgccacatc | 4000 |
| 4001 | ttgcgaatat | atgtgtagaa | actgccggaa | atcgtcgtgg | tattcactcc | 4050 |
| 4051 | agagcgatga | aaacgtttca | gtttgctcat | ggaaaacggt | gtaacaaggg | 4100 |
| 4101 | tgaacactat | cccatatcac | cagctcaccg | tctttcattg | ccatacggaa | 4150 |
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pBR329
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