Anti-Ryanodine Receptor 1

Anti-Ryanodine Receptor 1

Skeletal Muscle-Type Ryanodine Receptor, RyR1
Cat #: ARR-001
Sizes: 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IC, IH, WB
May also work in: IFC, IP
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Expression of RyR1 in rat tibialis anterior muscle.Immunohistochemical staining of rat tibialis anterior muscle sections using Anti-Ryanodine Receptor 1 antibody (#ARR-001). RyR1 co-localizes with Calpain II.Adapted from Kraner, S.D. et al. (2011) Am. J. Physiol. 300, R1384. with permission of The American Physiological Society.

Anti-Ryanodine Receptor 1 (#ARR-001) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize RyR1 from human, rat, and mouse samples.

For a list of product citations in the literature, see product citations tab below. If you know of additional relevant citations for this product, please let us know.

• Applications
• Specifications
• Scientific Background
• Citations
• Related Products

Western blot

Western blot analysis of rat skeletal muscle lysates:
1. Anti-Ryanodine Receptor 1 antibody (#ARR-001), (1:200).   
2. Anti-Ryanodine Receptor 1 antibody, preincubated with the control peptide antigen.
Immunohistochemistry

Expression of Ryanodine Receptor 1 in rat skeletal muscle
Immunohistochemical staining of paraffin embedded sections of rat quadriceps using Anti-Ryanodine Receptor 1 antibody (#ARR-001), (1:100). Ryanodine Receptor 1 is expressed in fibers of striate muscle. Note that smooth muscle in the arterial walls is negative as well as the surrounding connective tissue. Hematoxilin is used as the counterstain.

Expression of Ryanodine Receptor 1 in mouse cerebellum
Immunohistochemical staining of mouse cerebellum frozen sections using Anti-Ryanodine Receptor 1 antibody (#ARR-001), (1:100), (green). A. The highest expression of Ryanodine Receptor 1 is in the molecular layer (Asterisk). B. In the same section, there is staining for parvalbumin (red), a marker of Purkinje cells. C. Merged image of panels A and B demonstrates that Ryanodine Receptor 1 is localized surrounding the dendritic tree of Purkinje cells. DAPI is used as the counterstain.
Immunocytochemistry
HEK-293 cells transfected with rabbit RyR1 (1:500) (see Kaya, L. et al. (2013) Biochim. Piophys. Acta 1833, 1421.)
Immunogen
Peptide (C)RAENEKDATTEKNKKR, corresponding to amino acid residues 1371-1386 of human Ryanodine Receptor 1 (Accession P21817).  Intracellular, N-terminus.

HomologyRat, mouse - identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

It is well established that cytosolic calcium (Ca²⁺) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.^1,2  The primary intracellular Ca²⁺ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.

The ER and SR contain two Ca²⁺ release channels families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).³

The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).³ The Ryanodine receptors are homotetrameric proteins generating a Ca²⁺ conducting channel . They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine to the Ryanodine Receptor causes to two major changes in the channel: a reduction in single-channel conductance and a marked increase in open state probability.

The RyR1 is expressed predominantly in skeletal muscles and areas of the brain; the human RyR1 has at least three known alternative spliced variants.⁵

Several diseases are attributed to mutations in RyR1 gene: Central Core Disease (CCD), multi-minicore disease (MmD) and Malignant Hyperthermia (MH).⁵

References

1. Chakrabarti, R. And Chakrabarti, R. (2006) J.Cell. Biochem. 99, 1503.
2. Eisner, D.A. et al. (2005) Exp.Physiol. 90, 3.
3. Hamilton, S.L. (2005) Cell Calcium 38, 253.
4. Fill, M. and Copello, J.A. (2002) Physiol Rev. 82, 893.
5. UniProtKB/Swiss-Prot entry P21817 [RYR1_HUMAN] Ryanodine receptor 1.