Anti-Ryanodine Receptor 3

Anti-Ryanodine Receptor 3

Brain Ryanodine Receptor, RyR3
Cat #: ARR-003
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IC, IH, WB
May also work in: IFC, IP
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Expression of RyR3 in rat PASMCs.Immunocytochemical staining of rat pulmonary arterial smooth muscle cells (PASMCs) using Anti-Ryanodine Receptor 3 antibody (#ARR-003), (red). Cav-1 is stained in green.Adapted from Gilbert, G. et al. (2016) Am. J. Physiol. 310, L1078. with permission of The American Physiological Society.

Anti-Ryanodine Receptor 3 (#ARR-003) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize RyR3 from human, rat, and mouse samples.

For a list of product citations in the literature, see product citations tab below. If you know of additional relevant citations for this product, please let us know.

• Applications
• Specifications
• Scientific Background
• Citations
• Related Products

Western blot

Western blot analysis of rat brain membranes:
1. Anti-Ryanodine Receptor 3 antibody (#ARR-003), (1:200).
2. Anti-Ryanodine Receptor 3 antibody, preincubated with the control peptide antigen.
Immunohistochemistry

Expression of Ryanodine Receptor 3 in rat cortex
Immunohistochemical staining of rat brain frozen sections with Anti-Ryanodine Receptor 3 antibody (#ARR-003), (red), (1:100). Ryanodine Receptor 3 is widely expressed in neurons of the cortex. Hoechst 33342 is used as the counterstain.
Immunocytochemistry
Human ESC NPs (1:100) (see Forostyak, O. et al. (2013) Stem Cells. Dev. 22, 1506.).
Immunogen
Peptide CHIAHVNPAAEEDLK, corresponding to amino acid residues 935-949 of human Ryanodine Receptor 3 (Accession Q15413). Intracellular, N-terminus.

HomologyRat, mouse - identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.75 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

It is well established that cytosolic calcium (Ca²⁺) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.^1,2  The primary intracellular Ca²⁺ storage/release organelles in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.

The ER and SR contain two Ca²⁺ release channel families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).³

The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).³ The Ryanodine receptors are homotetrameric proteins generating a Ca²⁺ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine (poisonous alkaloid found in the South American plant Ryania speciosa to the Ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to an overall increase/decrease in the Ca²⁺ release capability of the channel.

RyR3 is the most widely expressed RyR channel which is predominantly expressed in smooth muscle tissues and certain regions of the brain.

References

1. Chakrabarti, R. And Chakrabarti, R. (2006) J.Cell. Biochem. 99, 1503.
2. Eisner, D.A. et al. (2005) Exp.Physiol. 90, 3.
3. Hamilton, S.L. (2005) Cell Calcium 38, 253
4. Fill, M. and Copello, J.A. (2002) Physiol. Rev. 82, 893.