Anti-KChIP2

Anti-KChIP2

K_v channel interacting protein 2, KCNIP2
Cat #: APC-142
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IH, WB
May also work in: IC, IFC, IP
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Anti-KChIP2 (#APC-142) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KChIP2 from rat, mouse, and human samples.

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of mouse brain lysate:
1. Anti-KChIP2 antibody (#APC-142), (1:200).
2. Anti-KChiP2 antibody, preincubated with the control peptide antigen.

Western blot analysis of rat heart lysate (lanes 1 and 3) and rat hippocampus lysate (lanes 2 and 4):
1, 2. Anti-KChIP2 antibody (#APC-142), (1:200).
3, 4. Anti-KChiP2 antibody, preincubated with the control peptide antigen.
Immunohistochemistry

Expression of KChIP2 in mouse hippocampus
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-KChIP2 antibody (#APC-142), (1:400), followed by goat-anti-rabbit-Alexa-488 (green). KChIP2 staining in the hippocampal CA1 region appears in neuronal profiles (arrows) in the pyramidal layer (Pyr). DAPI is used to stain nuclei (blue).
Immunogen
Peptide (C)RGQGRKESLSDSRD, corresponding to amino acid residues 2-15 of human KChIP2 (Accession Q9NS61). Cytoplasmic, N-terminus.

HomologyMouse - identical. Rat - 13/14 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

KChIPs belong to the family of small cytosolic calcium-binding proteins and consists of four different KChIP isoform ranging between 216 and 270 amino acids. They all have a reasonably conserved core region of 185 amino acids and a heterogeneous N-terminal. KChIPs have calcium-binding EF hands which appear important for modulation K_V channels but not for Ca_V channels¹.

The cardiac KChIP2 gene generates three protein isoforms of 220, 252, and 270 amino acids via alternative splicing of exons 2 and 3. It is well established that KChIP2 functionally increases peak K_V4 current, slows channel inactivation and accelerates recovery from inactivation. Furthermore, KChIP2 has been reported to increase I _{Ca ,L}  and suggested to decrease K_V1.5 cell surface expression and facilitate I_Na² .

All four KChIP mRNAs are found in the brain; however only KChIP2 is expressed in the heart³. KChIP2 is expressed heterogeneously across the canine and human left and right ventricular free wall with higher concentrations in epicardium compared to endocardium⁴.

Mice deficient for KChIP2 exhibit normal cardiac structure and function but display a prolonged elevation in the ST segment on the electrocardiogram. The KChIP2^−/− mice are highly susceptible to the induction of cardiac arrhythmias⁵.

References

1. Holmqvist, M.H. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 1035.
2. Lundby, A. et al. (2010) Br. J. Pharmacol. 160, 2028.
3. Rosati, B. et al. (2001) J. Physiol. (Lond.) 533, 119.
4. Grubb, S. et al. (2012) Front. Physiol. 3, 118.
5. Kuo, H.C. et al. (2001) Cell 107, 801.