lambda gt102(lambdagt102)

Deposited by: Jinseon Lee, Sherman M. Weissman Restriction digests of the clone give the following sizes (kb): HindIII–>23.0, 9.6, 6.6; BamHI–19.4, 12.0, 6.2, 6.0, 1.5; PstI–19.4, 6.0, 3.2, 2.7, 2.1, 1.8, 1.25, 1.2, 1.1, 0.95, 0.9, 0.8, 0.6, 0.5, 0.4, 0.3, 0.2, 0.2; EcoRI–33.5, 10.5. (ATCC staff) Cloning vector permitting positive selection of inserts and useful for construction of jumping clones and libraries. Does not cross-hybridize to sequences found in common plasmid vectors. [1] DNA inserted into the cloning sites interrupts the imm434 cI repressor, and thus plaque formation of nonrecombinants is repressed on high-frequency lysogeny mutant strains such as C600hflA or BNN102. [1] The genotype appears to be more stable in the cI434 region than lambdagt10. (personal communication) May not be distributed for commercial use. (personal communication) Differs from lambdagt101 (ATCC 77363) and lambdagt103 (ATCC 77365) only in the cI434 region. (personal communication) Medium is 1592 SM buffer. Hosts: E.coli, E.coli C600, E.coli BNN102, bacteria-free lysate.