- 询价
- TIANDZ
- ZK-0068550
- 中国
- 2025年11月20日
1 年钻石会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pET-41b(+)
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号: 60908-2807b
质粒名称: pET-41 b(+)
其他名称: pET41b, pET 41b
质粒大小（bp）: 5932
抗性: Kanamycin（卡那霉素）
质粒类型: Bacterial Expression（大肠杆菌蛋白表达）
是否病毒: 非病毒
启动子:
稳转或瞬转: Transient （瞬时表达）
组成型或诱导性: Constitutive （组成型）
表达水平: High
蛋白标签: N-GST, N-His, N-Thrombin
5′ 测序引物及序列: T7: 5′-TAATACGACTCACTATAGGG-3′; pGEX-5′: 5′-GGGCTGGCAAGCCACGTTTGGTG-3′
3’测序引物及序列：无
GenBank号:
其他属性: Notes: Encodes GST fusion tag; Nterm thrombin cleavage site; Nterm enterokinase cleavage site; PshAI blunt cloning site; a,b,c vary by MCS
Selectable Marker: Neomycin
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 120 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| EK | 278 | 264 | |
| S15 | 353 | 309 | |
| 6xHIS | 413 | 396 | |
| GST (variant) | 1094 | 444 | |
| T7_transl_en_RBS | 1119 | 1103 | |
| lacO | 1164 | 1137 | |
| T7_promoter | 1182 | 1164 | |
| tet (300 – 563) | 1218 | 1481 | |
| pBRrevBam_primer | 1289 | 1270 | |
| lacI | 1564 | 2655 | |
| ROP | 3227 | 3418 | |
| pGEX_3_primer | 3434 | 3412 | |
| pBR322_origin | 4452 | 3833 | |
| KanR2 | 4558 | 5373 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 3 | 1094 | 147 | |
| ORF frame 3 | 1095 | 1823 | |
| ORF frame 1 | 1696 | 2655 | |
| ORF frame 1 | 4558 | 5373 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 174 |
| NotI | 182 |
| EagI | 182 |
| HindIII | 189 |
| SalI | 195 |
| SacI | 206 |
| PstI | 214 |
| AscI | 216 |
| StuI | 226 |
| EcoRI | 235 |
| BamHI | 241 |
| EcoRV | 251 |
| NcoI | 254 |
| AgeI | 291 |
| KpnI | 298 |
| BglII | 301 |
| SacII | 392 |
| SpeI | 423 |
| MscI | 886 |
| NdeI | 1093 |
| XbaI | 1131 |
| ApaI | 2134 |
| HpaI | 2429 |
| NruI | 4646 |
| SmaI | 4863 |
| XmaI | 4861 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgtttagcag | cctaggtatt | aatcaattag | 150 |
| 151 | tggtggtggt | ggtggtggtg | gtgctcgagt | gcggccgcaa | gcttgtcgac | 200 |
| 201 | ggagctcgcc | tgcaggcgcg | ccaaggcctg | tacagaattc | ggatccccga | 250 |
| 251 | tatccatggg | actcttgtcg | tcgtcatcac | cggagccacc | accggtaccc | 300 |
| 301 | agatctgggc | tgtccatgtg | ctggcgttcg | aatttagcag | cagcggtttc | 350 |
| 351 | tttcatacca | attgcagtac | taccgcgtgg | caccagaccc | gcggagtgat | 400 |
| 401 | ggtgatggtg | atgaccagaa | ccactagttg | aaccatccga | ttttggagga | 450 |
| 451 | tggtcgccac | caccaaacgt | ggcttgccag | ccctgcaaag | gccatgctat | 500 |
| 501 | atacttgctg | gatttcaagt | acttatcaat | ttgtgggata | gcttcaatac | 550 |
| 551 | gttttttaaa | acaaactaat | tttgggaacg | catccaggca | cattgggtcc | 600 |
| 601 | atgtataaaa | caacatcaag | agcgtcatac | aacatgaagt | caggatgggt | 650 |
| 651 | tacatgatca | ccatttaaat | atgttttatg | acataaacga | tcttcgaaca | 700 |
| 701 | ttttcagcat | ttcaggtagc | ttgctaagaa | aatcaacttt | gagagtttca | 750 |
| 751 | aagtctttac | tatatgcaat | tctcgaaaca | ccgtatctaa | tatccaaaac | 800 |
| 801 | cgctccttca | agcattgaaa | tctctgcacg | ctcttttgga | caaccaccca | 850 |
| 851 | acatgttgtg | cttgtcagct | atataacgta | tgatggccat | agactgtgtt | 900 |
| 901 | aatttaacat | caccatcaat | ataataagga | agattgggaa | actccaaacc | 950 |
| 951 | caattcaaac | tttttgtttc | gccatttatc | accttcatcg | cgctcataca | 1000 |
| 1001 | aatgctcttc | atatttttct | tcaagatatt | ccaaaagaag | tcgagtgggt | 1050 |
| 1051 | tgcacaaggc | ccttaatttt | ccaataacct | agtatagggg | acatatgtat | 1100 |
| 1101 | atctccttct | taaagttaaa | caaaattatt | tctagagggg | aattgttatc | 1150 |
| 1151 | cgctcacaat | tcccctatag | tgagtcgtat | taatttcgcg | ggatcgagat | 1200 |
| 1201 | cgatctcgat | cctctacgcc | ggacgcatcg | tggccggcat | caccggcgcc | 1250 |
| 1251 | acaggtgcgg | ttgctggcgc | ctatatcgcc | gacatcaccg | atggggaaga | 1300 |
| 1301 | tcgggctcgc | cacttcgggc | tcatgagcgc | ttgtttcggc | gtgggtatgg | 1350 |
| 1351 | tggcaggccc | cgtggccggg | ggactgttgg | gcgccatctc | cttgcatgca | 1400 |
| 1401 | ccattccttg | cggcggcggt | gctcaacggc | ctcaacctac | tactgggctg | 1450 |
| 1451 | cttcctaatg | caggagtcgc | ataagggaga | gcgtcgagat | cccggacacc | 1500 |
| 1501 | atcgaatggc | gcaaaacctt | tcgcggtatg | gcatgatagc | gcccggaaga | 1550 |
| 1551 | gagtcaattc | agggtggtga | atgtgaaacc | agtaacgtta | tacgatgtcg | 1600 |
| 1601 | cagagtatgc | cggtgtctct | tatcagaccg | tttcccgcgt | ggtgaaccag | 1650 |
| 1651 | gccagccacg | tttctgcgaa | aacgcgggaa | aaagtggaag | cggcgatggc | 1700 |
| 1701 | ggagctgaat | tacattccca | accgcgtggc | acaacaactg | gcgggcaaac | 1750 |
| 1751 | agtcgttgct | gattggcgtt | gccacctcca | gtctggccct | gcacgcgccg | 1800 |
| 1801 | tcgcaaattg | tcgcggcgat | taaatctcgc | gccgatcaac | tgggtgccag | 1850 |
| 1851 | cgtggtggtg | tcgatggtag | aacgaagcgg | cgtcgaagcc | tgtaaagcgg | 1900 |
| 1901 | cggtgcacaa | tcttctcgcg | caacgcgtca | gtgggctgat | cattaactat | 1950 |
| 1951 | ccgctggatg | accaggatgc | cattgctgtg | gaagctgcct | gcactaatgt | 2000 |
| 2001 | tccggcgtta | tttcttgatg | tctctgacca | gacacccatc | aacagtatta | 2050 |
| 2051 | ttttctccca | tgaagacggt | acgcgactgg | gcgtggagca | tctggtcgca | 2100 |
| 2101 | ttgggtcacc | agcaaatcgc | gctgttagcg | ggcccattaa | gttctgtctc | 2150 |
| 2151 | ggcgcgtctg | cgtctggctg | gctggcataa | atatctcact | cgcaatcaaa | 2200 |
| 2201 | ttcagccgat | agcggaacgg | gaaggcgact | ggagtgccat | gtccggtttt | 2250 |
| 2251 | caacaaacca | tgcaaatgct | gaatgagggc | atcgttccca | ctgcgatgct | 2300 |
| 2301 | ggttgccaac | gatcagatgg | cgctgggcgc | aatgcgcgcc | attaccgagt | 2350 |
| 2351 | ccgggctgcg | cgttggtgcg | gacatctcgg | tagtgggata | cgacgatacc | 2400 |
| 2401 | gaagacagct | catgttatat | cccgccgtta | accaccatca | aacaggattt | 2450 |
| 2451 | tcgcctgctg | gggcaaacca | gcgtggaccg | cttgctgcaa | ctctctcagg | 2500 |
| 2501 | gccaggcggt | gaagggcaat | cagctgttgc | ccgtctcact | ggtgaaaaga | 2550 |
| 2551 | aaaaccaccc | tggcgcccaa | tacgcaaacc | gcctctcccc | gcgcgttggc | 2600 |
| 2601 | cgattcatta | atgcagctgg | cacgacaggt | ttcccgactg | gaaagcgggc | 2650 |
| 2651 | agtgagcgca | acgcaattaa | tgtaagttag | ctcactcatt | aggcaccggg | 2700 |
| 2701 | atctcgaccg | atgcccttga | gagccttcaa | cccagtcagc | tccttccggt | 2750 |
| 2751 | gggcgcgggg | catgactagc | atgatcgtgc | tcctgtcgtt | gaggacccgg | 2800 |
| 2801 | ctaggctggc | ggggttgcct | tactggttag | cagaatgaat | caccgatacg | 2850 |
| 2851 | cgagcgaacg | tgaagcgact | gctgctgcaa | aacgtctgcg | acctgagcaa | 2900 |
| 2901 | caacatgaat | ggtcttcggt | ttccgtgttt | cgtaaagtct | ggaaacgcgg | 2950 |
| 2951 | aagtcagcgc | cctgcaccat | tatgttccgg | atctgcatcg | caggatgctg | 3000 |
| 3001 | ctggctaccc | tgtggaacac | ctacatctgt | attaacgaag | cgctggcatt | 3050 |
| 3051 | gaccctgagt | gatttttctc | tggtcccgcc | gcatccatac | cgccagttgt | 3100 |
| 3101 | ttaccctcac | aacgttccag | taaccgggca | tgttcatcat | cagtaacccg | 3150 |
| 3151 | tatcgtgagc | atcctctctc | gtttcatcgg | tatcattacc | cccatgaaca | 3200 |
| 3201 | gaaatccccc | ttacacggag | gcatcagtga | ccaaacagga | aaaaaccgcc | 3250 |
| 3251 | cttaacatgg | cccgctttat | cagaagccag | acattaacgc | ttctggagaa | 3300 |
| 3301 | actcaacgag | ctggacgcgg | atgaacaggc | agacatctgt | gaatcgcttc | 3350 |
| 3351 | acgaccacgc | tgatgagctt | taccgcagct | gcctcgcgcg | tttcggtgat | 3400 |
| 3401 | gacggtgaaa | acctctgaca | catgcagctc | ccggagacgg | tcacagcttg | 3450 |
| 3451 | tctgtaagcg | gatgccggga | gcagacaagc | ccgtcagggc | gcgtcagcgg | 3500 |
| 3501 | gtgttggcgg | gtgtcggggc | gcagccatga | cccagtcacg | tagcgatagc | 3550 |
| 3551 | ggagtgtata | ctggcttaac | tatgcggcat | cagagcagat | tgtactgaga | 3600 |
| 3601 | gtgcaccata | tatgcggtgt | gaaataccgc | acagatgcgt | aaggagaaaa | 3650 |
| 3651 | taccgcatca | ggcgctcttc | cgcttcctcg | ctcactgact | cgctgcgctc | 3700 |
| 3701 | ggtcgttcgg | ctgcggcgag | cggtatcagc | tcactcaaag | gcggtaatac | 3750 |
| 3751 | ggttatccac | agaatcaggg | gataacgcag | gaaagaacat | gtgagcaaaa | 3800 |
| 3801 | ggccagcaaa | aggccaggaa | ccgtaaaaag | gccgcgttgc | tggcgttttt | 3850 |
| 3851 | ccataggctc | cgcccccctg | acgagcatca | caaaaatcga | cgctcaagtc | 3900 |
| 3901 | agaggtggcg | aaacccgaca | ggactataaa | gataccaggc | gtttccccct | 3950 |
| 3951 | ggaagctccc | tcgtgcgctc | tcctgttccg | accctgccgc | ttaccggata | 4000 |
| 4001 | cctgtccgcc | tttctccctt | cgggaagcgt | ggcgctttct | catagctcac | 4050 |
| 4051 | gctgtaggta | tctcagttcg | gtgtaggtcg | ttcgctccaa | gctgggctgt | 4100 |
| 4101 | gtgcacgaac | cccccgttca | gcccgaccgc | tgcgccttat | ccggtaacta | 4150 |
| 4151 | tcgtcttgag | tccaacccgg | taagacacga | cttatcgcca | ctggcagcag | 4200 |
| 4201 | ccactggtaa | caggattagc | agagcgaggt | atgtaggcgg | tgctacagag | 4250 |
| 4251 | ttcttgaagt | ggtggcctaa | ctacggctac | actagaagga | cagtatttgg | 4300 |
| 4301 | tatctgcgct | ctgctgaagc | cagttacctt | cggaaaaaga | gttggtagct | 4350 |
| 4351 | cttgatccgg | caaacaaacc | accgctggta | gcggtggttt | ttttgtttgc | 4400 |
| 4401 | aagcagcaga | ttacgcgcag | aaaaaaagga | tctcaagaag | atcctttgat | 4450 |
| 4451 | cttttctacg | gggtctgacg | ctcagtggaa | cgaaaactca | cgttaaggga | 4500 |
| 4501 | ttttggtcat | gaacaataaa | actgtctgct | tacataaaca | gtaatacaag | 4550 |
| 4551 | gggtgttatg | agccatattc | aacgggaaac | gtcttgctct | aggccgcgat | 4600 |
| 4601 | taaattccaa | catggatgct | gatttatatg | ggtataaatg | ggctcgcgat | 4650 |
| 4651 | aatgtcgggc | aatcaggtgc | gacaatctat | cgattgtatg | ggaagcccga | 4700 |
| 4701 | tgcgccagag | ttgtttctga | aacatggcaa | aggtagcgtt | gccaatgatg | 4750 |
| 4751 | ttacagatga | gatggtcaga | ctaaactggc | tgacggaatt | tatgcctctt | 4800 |
| 4801 | ccgaccatca | agcattttat | ccgtactcct | gatgatgcat | ggttactcac | 4850 |
| 4851 | cactgcgatc | cccgggaaaa | cagcattcca | ggtattagaa | gaatatcctg | 4900 |
| 4901 | attcaggtga | aaatattgtt | gatgcgctgg | cagtgttcct | gcgccggttg | 4950 |
| 4951 | cattcgattc | ctgtttgtaa | ttgtcctttt | aacagcgatc | gcgtatttcg | 5000 |
| 5001 | tctcgctcag | gcgcaatcac | gaatgaataa | cggtttggtt | gatgcgagtg | 5050 |
| 5051 | attttgatga | cgagcgtaat | ggctggcctg | ttgaacaagt | ctggaaagaa | 5100 |
| 5101 | atgcataaac | ttttgccatt | ctcaccggat | tcagtcgtca | ctcatggtga | 5150 |
| 5151 | tttctcactt | gataacctta | tttttgacga | ggggaaatta | ataggttgta | 5200 |
| 5201 | ttgatgttgg | acgagtcgga | atcgcagacc | gataccagga | tcttgccatc | 5250 |
| 5251 | ctatggaact | gcctcggtga | gttttctcct | tcattacaga | aacggctttt | 5300 |
| 5301 | tcaaaaatat | ggtattgata | atcctgatat | gaataaattg | cagtttcatt | 5350 |
| 5351 | tgatgctcga | tgagtttttc | taagaattaa | ttcatgagcg | gatacatatt | 5400 |
| 5401 | tgaatgtatt | tagaaaaata | aacaaatagg | ggttccgcgc | acatttcccc | 5450 |
| 5451 | gaaaagtgcc | acctgaaatt | gtaaacgtta | atattttgtt | aaaattcgcg | 5500 |
| 5501 | ttaaattttt | gttaaatcag | ctcatttttt | aaccaatagg | ccgaaatcgg | 5550 |
| 5551 | caaaatccct | tataaatcaa | aagaatagac | cgagataggg | ttgagtgttg | 5600 |
| 5601 | ttccagtttg | gaacaagagt | ccactattaa | agaacgtgga | ctccaacgtc | 5650 |
| 5651 | aaagggcgaa | aaaccgtcta | tcagggcgat | ggcccactac | gtgaaccatc | 5700 |
| 5701 | accctaatca | agttttttgg | ggtcgaggtg | ccgtaaagca | ctaaatcgga | 5750 |
| 5751 | accctaaagg | gagcccccga | tttagagctt | gacggggaaa | gccggcgaac | 5800 |
| 5801 | gtggcgagaa | aggaagggaa | gaaagcgaaa | ggagcgggcg | ctagggcgct | 5850 |
| 5851 | ggcaagtgta | gcggtcacgc | tgcgcgtaac | caccacaccc | gccgcgctta | 5900 |
| 5901 | atgcgccgct | acagggcgcg | tcccattcgc | ca | 5932 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
pET-41b(+)
询价
