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- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
Recombinant Apoptotic Peptidase Activating Factor 1 (APAF1)
- 保质期:
12个月
- 供应商:
上海再康
- 保存条件:
-20℃
| Organism species | Homo sapiens (Human) |
| Product No. | zk054Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 39.6kDa |
| Concentration | 9.0 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SDGFLTISEE EKVRNEPTQQ QRAAMLIKMI LKKDNDSYVS FYNALLHEGY KDLAALLHDG IPVVSSSSGK DSVSGITSYV RTVLCEGGVP QRPVVFVTRK KLVNAIQQKL SKLKGEPGWV TIHGMAGCGK SVLAAEAVRD HSLLEGCFPG GVHWVSVGKQ DKSGLLMKLQ NLCTRLDQDE SFSQRLPLNI EEAKDRLRIL MLRKHPRSLL ILDDVWDSWV LKAFDSQCQI LLTTRDKSVT DSVMGPKYVV PVESSLGKEK GLEILSLFVN MKKADLPEQA HSIIKECKGS PLVVSLIGAL LRDFPNRWEY YLKQLQNKQF
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
凋亡蛋白酶激活因子1(APAF1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验of caspase),Cyt C通过Apaf-1因子的多聚化与胱天蛋白酶(caspases)-9形成凋亡小体,导致下游胱天蛋白酶的级联反应。而凋亡蛋白抑制因子(IAP)和Smac通过抑制和促进胱天蛋白酶的级联反应来调控细胞凋亡。 The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and the release of cytochrome C. The anti
Translation.Blood,2026 May 12 图源:10.1182/blood.2026034144[2] City of Hope的李凌团队在Blood发表了一篇研究。该研究通过系统筛选发现PRMT9在急性髓系白血病(AML)维奈托克耐药中发挥关键作用,揭示了其通过调控RNA剪接和蛋白翻译双重机制驱动耐药的新模式。机制上,PRMT9抑制导致ALG13基因发生关键剪接异常,进而下调药物外排转运蛋白ABCC1表达;同时PRMT9抑制降低整体翻译活性,使短寿命抗凋亡蛋白MCL1显著下调。功能
体,从而激活凋亡。 5、FasL 也是一个单一跨膜蛋白。FasL 既可通过与之相互作用杀死相邻细胞,也可激活细胞自身的Fas而自杀。 (二)Apaf1- caspase活化蛋白 1、Caspase-8裂解 Bid 使其 C-端域易位至线粒体中。 2、Bid及其它 Bcl2家族促凋亡蛋白的作用导致线粒体释放细胞色素C。. 3、Bcl2抑制cytochrome c释放。 4、Cytochrome c 在胞浆中与Apaf-1结合。Apaf-1/cytochrome c经需能的寡聚化形成大的蛋白
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