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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
Recombinant D Site Of Albumin Promoter Binding Protein (DBP)
- 保质期:
12个月
- 供应商:
上海再康
- 保存条件:
-20℃
| Organism species | Rattus norvegicus (Rat) |
| Product No. | zk292Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
白蛋白启动子D位点结合蛋白(DBP)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验也可以从其他物种中去除白蛋白。适用于处理 25 或 50 个样本。Figure 1: 2D analysis of whole human serum before (left) and after(right) treatment with AlbminOUT™. 产品特点: ● 从样本中不到 10 分钟的时间内去除白蛋白 ● 基于白蛋白和 Cibachron 蓝色染料的结合 ● 每个柱子的结合力大于 2 mg 的人白蛋白 ● 从 5-50
的表达系统会造成质粒的不稳定,细胞生长速度的下降和重组蛋白产量的降低[32,33]。Lanzer和Bujard曾对常用的lac启动子-操纵子系统进行了广泛的研究,证明操纵子放在启动子序列的不同位置会造成70倍差异的抑制。将17bp的操纵子置于-10和-35六聚体区之间所形成的抑制比将其放在-35区的上游或-10区的下游要高50-70倍[34]。 启动子的第三个特性是其简便和廉价的可诱导性。大量生产蛋白质最常用的启动子是热诱导(λPL)和化学诱导(trp)启动子。异丙基硫代-β-D-半乳糖
子与启动子序列的距离缩短(在2kb范围内),加强了增强子对启动子的顺式激活作用(cisacting)。在淋巴细胞特异性DNA结合蛋白(DBP)如OTF2A、OTF2B作用下,增强子可促进Ⅱ型RNA聚合酶与可变区基因上游5,端的启动子高保守序列TATAb。x结合,从而启动/z基因的转录,产生初级mRNA。转录的初级mRNA中仍然含有内含子等非编码序列,需经mRNA 5’端加帽、3,端多聚腺苷酸化、甲基化等碱基修饰、mRNA拼接(内含子切除)等转录后加工,成熟的mRNA从细胞核释放出来,随后在核糖体上进
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