相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
Recombinant C-Src Tyrosine Kinase (CSK)
- 保质期:
12个月
- 供应商:
上海再康
- 保存条件:
-20℃
| Organism species | Homo sapiens (Human) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 25.8kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Leu243~Glu438 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
LRHSNLVQ LLGVIVEEKG GLYIVTEYMA KGSLVDYLRS RGRSVLGGDC LLKFSLDVCE AMEYLEGNNF VHRDLAARNV LVSEDNVAKV SDFGLTKEAS STQDTGKLPV KWTAPEALRE KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDAPDG CPPAVYEVMK NCWHLDAAMR PSFLQLRE
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验(src) FcεR Ⅱ(CD23) 肥大细胞、嗜碱性粒细胞 Fyn CD36 血小板 Lyn、Fyn、Yes 一、p56 lck p56 lck 属于以p60v-src/c-src为代表的蛋白酪氨酸激酶家族成员。同这个家族的其它成员一样都具有酪氨酸激酶活性。目前发现,p56 lck 相对特异地存在于淋巴细胞中,尤其是成熟的静止T淋巴细胞中。p56 lck 可能在T
CD45 分子参与启动信号转导 然而事实上,上述过程并不产生。Src分子有两个酪氨酸残基位点,一个位于催化结构域即SHl内394位置处,是激酶显示活性的关键部位,也是相互磷酸化中Src分子间磷酸根转移的靶目标。另一个酪氨酸残基在Sre分子C端505位置,发挥抑制作用。在未激活的T细胞中组成性表达一种称为Csk的蛋白酪氨酸激酶(图9―1)。Csk指C端Src激酶(C―terminal Srckinase),专以Y505为靶目标,使其一直处于磷酸化状态(成为
, Stuttgart, Germany) 。 ( 9 ) 成像板(BAS-SR 2025,Fujifilm,Japan) 。 ( 10 ) 磷屏显像仪(BAS-Reader-5000,Fujifilm) 。4. 注释 ( 1 ) 我们建议在激酶中加入甘油 [ 终浓度为 20% ( V/V)] 后保存在 -80°C,或将激酶在合适的缓冲液环境中冷冻干燥后保存在 -80°C,使用前,冻干的激酶要先用 ddH2O 溶解。 ( 2 ) 在进行基于芯片的激酶实验前,我们建议对激酶制备物进行胶内活性
技术资料暂无技术资料 索取技术资料





