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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 英文名：
Recombinant Splicing Factor 3B Subunit 3 (SF3B3)
- 保质期：
12个月
- 供应商：
上海再康
- 保存条件：
-20℃
剪接因子3B亚基3(SF3B3)重组蛋白
Recombinant Splicing Factor 3B Subunit 3 (SF3B3)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | zk216Mu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | >95% |
| UOM | 50ug |
| Predicted Molecular Mass | 23.1kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
剪接因子3B亚基3(SF3B3)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
GKILEL LRPDPNTGKV HTLLTVEVFG VIRSLMAFRL TGGTKDYIVV GSDSGRIVIL EYQPSKNMFE KIHQETFGKS GCRRIVPGQF LAVDPKGRAV MISAIEKQKL VYILNRDAAA RLTISSPLEA HKANTLVYHV VGVDVGFENP MFACLEMDYE EADNDPTGEA AANTQQTLTF
GKILEL LRPDPNTGKV HTLLTVEVFG VIRSLMAFRL TGGTKDYIVV GSDSGRIVIL EYQPSKNMFE KIHQETFGKS GCRRIVPGQF LAVDPKGRAV MISAIEKQKL VYILNRDAAA RLTISSPLEA HKANTLVYHV VGVDVGFENP MFACLEMDYE EADNDPTGEA AANTQQTLTF
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
剪接因子3B亚基3(SF3B3)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
剪接因子3B亚基3(SF3B3)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
剪接因子3B亚基3(SF3B3)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
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，并且人们对此知之甚少。17S U2 被认为是直接参与早期剪接体组装的人类 U2 snRNP 的功能形式。17S U2 snRNP 的核心成分包括 SF3b 配合物、SF3a 配合物、12S U2 核心、剪接因子 TAT-SF1 和 DDX46。体外实验也表明，SF3b 与 12S U2 核依次组装，形成一种被称为 15S 粒子的中间产物，最终形成 SF3a 复合物。然而，对组装这一过程的蛋白质因子却知之甚少。 最新的研究表明，具有 RNA 伴侣活性的 DDX42 可能是在 U2 snRNP 组装
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剪接因子3B亚基3(SF3B3)重组蛋白
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