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- ZK-0006505
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- 2025年11月20日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 英文名：
Cardiac myosin protein
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- Cardiac myosin protein详细信息：
- Product Uses Include
- Drug discovery target in cardiac research
- Measurement of F-actin activated myosin ATPase activity
- Identification/characterization of proteins or small molecules that affect myosin II ATPase activity
- Identification/characterization of proteins or small molecules that affect the myosin II - F-actin interaction
- Control myosin ATPase activity in drug discovery screening projects
Cardiac myosin proteinMaterial
Cardiac myosin protein has been purified from bovine heart muscle. The full length myosin protein has been purified with its essential light chains (ELC) and regulatory light chains (RLC), see Figure 1 and 2. Cardiac myosin has been determined to be biologically active in an F-actin activated ATPase assay, but it is not recommended for use in motility assays because of auto-inhibition (Protease digests with alpha-chymotrypsin and papain may create a subfragment that has F-actin moving properties. Cardiac myosin protein is supplied as a white lyophilized powder. When reconstituted in nanopure water, the protein will be in the following buffer: 100
mM Imidizole pH 7.5, 2.4 M KCl, 0.2 mM DTT, 5% sucrose and 1% dextran. The lyophilized protein is stable at 4°C desiccated (<10% humidity) for 1 year.
Cardiac myosin protein
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文献和实验Myofilament Protein Phosphorylation by PKC in Genetically Engineered Adult Cardiac Myocytes
in myocytes now makes it possible to investigate the relative phosphorylation of a given contractile protein as it resides within the intact myofilament. Contractile proteins, including troponin I (TnI), troponin T (TnT), myosin light chain 2 (MLC
Evaluation of the Cardiac Isoform of 2-Macroglobulin as a Factor Inducing Cardiac Hypertrophy
of cardiac hypertrophy. It was accompanied by enlargement of cardiac myocytes and induction of -myosin heavy chain (MHC) and MLC-2 gene expression. Multiple injections of 182-kDa protein-specific polyclonal antibody into the circulation of aorta-constricted
Myosin Light Chain Preparation
) For Cardiac Muscle- (1.5 liters ETOH 95% (1.5 liters H2O,3 ml 15 M MCE) Buffer E.Column Buffer: 16 liters: 20 mM Tris,15 mM MCE (160 ml 2 M Tris,16 ml 15 M MCE) Buffer F.Elution Buffer: 4 liters: 0.150 M KCl,10 mM Tris,15 mM MCE(150 ml 4 M KCl,40 ml 2 M
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