- 询价
- R&D
- ZK-0002898
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- Neurofilament NF-M Polyclonal Antibody, Purified保存于运输说明：
- Store at -20°C
- 详细信息：
-
Description Polyclonal Antibody against Neurofilament NF-M Form Purified Antibody Host Chicken Species Reactivity Human, Rat, Mouse, Cat IsoType IgY [Ab] Neurofilament NF-M Polyclonal Antibody, Purified2.6 mg/mL Specificity Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins, known as NF-L, NF-M and NF-H. Neurofilaments may also include smaller amounts of peripherin, α-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.
This antibody was raised in chickens against purified bacterially expressed C-terminal extension of rat NF-M, the so-called KE segment.Uses Neurofilament NF-M Polyclonal Antibody, PurifiedThis antibody is effective in immunoblotting and immunofluorescence. This antibody does not bind to protein A or protein G. Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:10,000*
- Immunofluorescence: 1:2,000
Notes * Predicted MW = 145-160kD Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Neurofilament NF-M Polyclonal Antibody, Purified
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文献和实验相关实验
Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
make an Antibody against a Peptides
. KLH induces a strong antibody response because of its large mass (>10 6 M.W.) and because it is a non-mammalian protein. Pierce recently introduced mariculture KLH (mcKLH), a product that is less damaging to marine aquatic environments
(either undiluted or 1:1 with PBT) or diluted polyclonal antiserum and incubate at 4 C overnight 8. Rinse with PBT 3 times, then wash 4 times for 15 minutes each 9. Add secondary antibody (usually 1:200 in PBT) and incubate 2 hours at room temperature
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Neurofilament NF-M Polyclonal Antibody, Purified
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