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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
Recombinant Otoraplin (OTOR)
- 保质期:
12个月
- 供应商:
上海再康
- 保存条件:
-20℃
| Organism species | Mus musculus (Mouse) |
| Product No. | zkC699Mu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 15.5kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Ala17~Thr121 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
AGHG VFMDKLSSKK LCADEECVYT ISLARAQEDY NAPDCRFIDV KKGQQIYVYS KLVTENGAGE FWAGSVYGDH QDEMGIVGYF PSNLVKEQRV YQEATKEIPT T
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验细胞因子简介 细胞因子(Cytokine,CK)是一类能在细胞间传递信息、具有免疫调节和效应功能的天然活性蛋白质,可以有效促进免疫细胞(如DC细胞,CIK细胞等)、干细胞的体外生长。根据细胞因子主要的功能可分为以下几类: 1,白细胞介素(Interleukin,IL)由淋巴细胞、单核细胞或其它非单个核细胞产生的细胞因子,在细胞间相互作用、免疫调节、造血以及炎症过程中起重要调节作用。 2,集落刺激因子(Colonystimulatingfactor,CSF)根据不同细胞因子刺激造血
-Triton蛋白分级抽提试剂盒细胞膜NP-40 or RIPA膜蛋白抽提试剂盒蛋白分级抽提试剂盒细胞核RIPA核蛋白抽提试剂盒线粒体RIPA线粒体蛋白抽提试剂盒亚细胞定位蛋白抽提试剂盒蛋白酶磷酸酶抑制剂抑制剂蛋白酶或磷酸酶PMSF丝氨酸蛋白酶抑制剂,如胰凝乳蛋白酶、胰蛋白酶和凝血酶,也抑制半胱氨酸蛋白酶如木瓜蛋白酶Leupeptin 亮肽素抑制丝氨酸和半胱氨酸蛋白酶,如胰蛋白酶、木瓜蛋白酶、纤溶酶和组织蛋白酶BAprotinin 抑肽酶丝氨酸蛋白酶抑制剂,抑制纤维蛋白溶酶、激肽释放酶、胰蛋白
。这一问题也使得比较不同研究者的实验数据极为困难。 血清中蛋白含量大且成分复杂(大于100种),使得实验和生产的标准化困难,使疫苗、单克隆抗体及生物活性蛋白等用培养细胞生产的生物产品的下游分离和纯化更复杂。干细胞培养要求在促进细胞增殖的同时,又要保持细胞的发育特性,不发生分化,对培养基组分的要求更加苛刻。使用血清可能改变细胞在体内的正常状态,可能促进某种细胞(成纤维细胞)生长的同时,抑制另一类细胞生长(表皮细胞)。在培养原代细胞时使用含血清的培养基可能不能阻止成纤维细胞过度
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