Lyo-Ready Probe可冻干预混液

概述​：

Lyo-Ready Probe一步法试剂盒是一种可冻干的RT-qPCR试剂盒，专为RNA序列的超灵敏检测而设计。这些特殊配制的预混液是开发诊断检测方法的理想选择。试剂盒包含无甘油的4x qPCR预混液，其组分包括热启动Taq DNA聚合酶、dNTPs、MgCl₂以及一系列赋形剂混合物，旨在确保冻干后功能最佳且活性无损失。​

​特点：​

1. RNA序列的超灵敏检测

2. 冻干后仍能提供与我们标准试剂盒相同的高性能

3. 包含无甘油的4x qPCR预混液

4. 预混了赋形剂，可可靠地冻干成珠状或饼状

5. 附带独立包装的高浓度UltraScript®逆转录酶（含RNase抑制剂）

6. 已验证可用于SARS-CoV-2核酸的定性检测

7. 适用于高通量、高度多重化的检测

8. 4x qPCR预混液也可单独购买用于DNA检测

​详细描述​

Lyo-Ready Probe一步法试剂盒经过特殊配制，用于使用探针法RT-qPCR超灵敏检测RNA，是开发冻干诊断测试的理想选择。

​为冻干工艺优化​

冻干具有许多优势，包括可常温储存、延长保质期以及增加样品体积的灵活性。该试剂盒经过优化，确保冻干后能提供与我们标准一步法RT-qPCR试剂盒相同的高性能。

​图1. 在多重检测中，冻干后性能优于竞争对手混合液​

​说明​：使用Lyo-Ready Probe一步法试剂盒（紫色曲线）与竞争对手M的一步法混合液（蓝色曲线）在完全冻干并用水复溶后，对常见冬季病毒（甲型流感、乙型流感、呼吸道合胞病毒和SARS-CoV-2）进行多重扩增。左侧面板显示Ct值，右侧面板显示扩增效率。使用了4个系列稀释的RNA模板，分别对应每个病毒基因组的4000、400、40和4个拷贝。总反应体积为20μL。循环条件为：45°C 20分钟，95°C 3分钟，然后进行50个循环的95°C 15秒，60°C 30秒。与竞争对手M相比，Lyo-Ready Probe一步法试剂盒在冻干后显示出更好的效率和灵敏度。

​稳健可靠的RT-qPCR​

Lyo-Ready Probe一步法试剂盒包含无甘油的4x qPCR预混液，其中含有热启动Taq DNA聚合酶、dNTPs、MgCl₂以及赋形剂混合物，以确保可靠的冻干过程，且无活性损失。抗体介导的热启动技术确保病毒来源cDNA的特异性扩增，并对临床样本中常见的PCR抑制剂具有更好的耐受性。UltraScript®逆转录酶以独立的高浓度3200x形式提供。这种经过改良的MMLV逆转录酶具有稳健性和热稳定性，经过工程化改造可提高cDNA合成速度和产量，并确保转录本表示的准确性，能够在高达55°C的温度下实现快速高效的逆转录。

​Better performance after lyophilisation in a multiplex setup
compared to a competitor mix: Multiplex amplification of common winter viruses (Influenza A, Influenza B, Respiratory Syncytial Virus, and SARS-CoV-2) using Lyo-Ready Probe 1-Step Kit (purple curves) or the 1-Step mix from Competitor M (blue curves) after complete lyophilisation andreconstitution with water. Ct values are shown in the left panels and efficiency in the right panels. 4 serial dilutions of RNA template were used, corresponding to 4000, 400, 40 and 4 copies of each viral genome. The total reaction volume was 20 μL. Cycle conditions were 45 C 20 min, 95 C 3 min and 50 cycles of 95 C 15 s, 60  C 30 s. Lyo-Ready Probe 1-Step Kit shows a better efficiency and sensitivity after
lyophilisation compared to Competitor M.

图2. 在多重检测中，冻干后性能优于竞争对手混合液​

​说明​：使用Lyo-Ready Probe一步法试剂盒（紫色柱）与竞争对手M的一步法混合液（蓝色柱）在完全冻干并用水复溶后，对常见冬季病毒进行多重扩增。顶部面板显示Ct值，底部面板显示效率。实验条件同图1。Lyo-Ready Probe一步法试剂盒在冻干后显示出更好的效率和灵敏度。

Better performance after lyophilisation in a multiplex setup compared to a competitor mix: Multiplex amplification of common winter viruses (Influenza A, Influenza B, Respiratory Syncytial Virus, and SARS-CoV-2) using Lyo-Ready Probe 1-Step Kit (purple bars) or the 1-Step mix from Competitor M (blue bars) after complete lyophilisation and reconstitution with water. Ct values are shown in the top panels and efficiency in the bottom panels. 4 serial dilutions of RNA template were used, corresponding to 4000, 400, 40 and 4 copies of each viral genome. The total reaction volume was 20 μL. Cycle conditions were 45 °C 20 min, 95 °C 3 min and 50 cycles of 95 °C 15 s, 60 °C 30 s. Lyo-Ready Probe 1-Step Kit shows a better efficiency and sensitivity after lyophilisation compared to Competitor M.

图3. 在多重检测中，冻干后性能可靠​

​说明​：使用Lyo-Ready Probe一步法试剂盒作为液体预混液（蓝色曲线）或作为冻干后用水复溶的混合液（紫色曲线），对常见冬季病毒进行多重扩增。左侧面板显示Ct值，右侧面板显示效率。实验条件同图1。Lyo-Ready Probe一步法试剂盒在冻干后未显示出效率或灵敏度的显著损失。

​Reliable performance after lyophilisation in a multiplex setup: Multiplex amplification of common winter viruses (Influenza A, Influenza B, Respiratory Syncytial Virus, and SARS-CoV-2) using the Lyo-Ready Probe 1-Step Kit as a wet mix (blue curves) or as a reconstituted mix with water after lyophilisation (purple curves).
Ct values are shown in the left panels and efficiency in the right panels. 4 serial dilutions of RNA template were used, corresponding to 4000, 400, 40 and 4 copies of each viral genome. The total reaction volume was 20 μL. Cycle conditions were 45 °C 20 min, 95 °C 3 min and 50 cycles of 95 °C 15 s, 60 °C 30 s. Lyo-Ready Probe 1-Step Kit shows no significant loss of efficiency or sensitivity after lyophilisation.

图4. 在多重检测中，冻干后性能可靠​

​说明​：使用Lyo-Ready Probe一步法试剂盒作为液体预混液（蓝色柱）或作为冻干后用水复溶的混合液（紫色柱），对常见冬季病毒进行多重扩增。顶部面板显示Ct值，底部面板显示效率。实验条件同图1。Lyo-Ready Probe一步法试剂盒在冻干后未显示出效率或灵敏度的显著损失。Reliable performance after lyophilisation in a multiplex setup: Multiplex amplification of common winter viruses (Influenza A, Influenza B, Respiratory Syncytial Virus, and SARS-CoV-2) using the Lyo-Ready Probe 1-Step Kit as a wet mix (blue bars) or as a reconstituted mix with water after lyophilisation (purple bars). Ct values are shown in the top panels and efficiency in the bottom panels. 4 serial dilutions of RNA template were used, corresponding to 4000, 400, 40 and 4 copies of each viral genome. The total reaction volume was 20 μL. Cycle conditions were 45  C 20 min, 95  C 3 min and 50 cycles of 95  C 15 s, 60  C 30 s. Lyo-Ready Probe 1-Step Kit shows no significant loss of efficiency or sensitivity after lyophilisation.