重组 HMPV (CAN97-83) Fusion 蛋白 (His & AVI Tag), Biotinylated

重组 HMPV (CAN97-83) Fusion 蛋白 (His & AVI Tag), Biotinylated（产品说明）
蛋白名称：HMPV (CAN97-83) Fusion Protein (His & AVI Tag), Biotinylated (MALS-verified)
蛋白构建：A DNA sequence encoding the HMPV (strain CAN97-83) Fusion glycoprotein (Q6WB98) with the pre-fusion trimer design was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS.
蛋白活性：1.Immobilized Recombinant HMPV (CAN97-83) Fusion Protein (His & AVI Tag), Biotinylated (Cat: 40993-V49H1-B) at 1 μg/mL (100 μl/well) can bind Anti-HMPV-F (Research Grade Clone SAN32-2), the EC50 is 0.6-1.8 ng/mL (QC tested). 2.Immobilized Recombinant HMPV (CAN97-83) Fusion Protein (His & AVI Tag), Biotinylated (Cat: 40993-V49H1-B) at 1 μg/mL (100 μl/well) can bind Anti-HMPV-F (Research Grade Clone MPV458), the EC50 is 1.1-3.3 ng/mL (Routinely tested). 3.Immobilized Anti-HMPV Antibody (MPE8) at 2 μg/mL (100 μl/well) can bind Recombinant HMPV (CAN97-83) Fusion Protein (His & AVI Tag), Biotinylated (Cat: 40993-V49H1-B), the EC50 is 7.5-21.5 ng/mL (Routinely tested). 4.Immobilized Anti-HMPV Antibody (SAN27-14)at 2 μg/mL (100 μl/well) can bind Recombinant HMPV (CAN97-83) Fusion Protein (His & AVI Tag), Biotinylated (Cat: 40993-V49H1-B), the EC50 is 3.5-10.5 ng/mL (Routinely tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Leu 19
蛋白分子量：The recombinant HMPV (HMPV)(strain CAN97-83) Fusion glycoprotein comprises 538 amino acids and has a predicted molecular mass of 58.96 kDa. It migrates as an approximately 64.7,55.2 and 16.7 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 226.5 kDa verified by SEC-MALS.
蛋白NP号：Q6WB98