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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
储存-20℃,干燥、避光、密封
- 保质期:
2年
- 英文名:
Ethyl (E)-3-(5-fluoro-1H-indol-3-yl)prop-2-enoate
- 库存:
1000
- 供应商:
四川省维克奇生物科技有限公司
- CAS号:
1649983-34-5
- 规格:
20mg
中文名称:(E)-3-(5-氟-1H-吲哚-3-基)丙-2-烯酸乙酯
英文名称:Ethyl (E)-3-(5-fluoro-1H-indol-3-yl)prop-2-enoate
分子式:C13H12FNO2
纯度:≥95%
CAS号:1649983-34-5
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文献和实验1.O/N (5ml E.coli in LB-->1ml into 200ml LB/37℃ 2.Grow 2-3H to A660 ~0.3-0.4 3.Chill cells 10'/ice Spin down 10',5K,4℃ 4.Wash 1X 200ml 10% glycerol (sterile)40℃; Spin down 10',5K,4℃ 5.Wash 1X 40 ml 10% glycerol--> transfer to S34 tubes & spin
Maxiprep of plasmid DNA from E. coli
Grow a single colony of E. coli overnight in 50mL LB broth + selective markers at 37°C. The next morning, put 850μL of the culture in each of two Eppendorf tubes, add 150μL sterile 50% glycerol, and store at -80°C. Pour as much culture as will fit
E.Z.N.A.® Mag-Bind® Viral DNA/RNA Spin Protocol
实验原理 If using the E.Z.N.A.™ Mag-Bind® Viral DNA/RNA Kit for the first time, please read this booklet to become familiar with the procedure and its various modification. Samples are lysed in a specially formulated buffer
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