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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
10 ml/20 ml
| 规格: | 10 ml | 产品价格: | ¥950.0 |
|---|---|---|---|
| 规格: | 20 ml | 产品价格: | ¥1500.0 |
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
4 in 1 liter H20 Fix in 0.5% glutaraldehyde in PBS + Mg2+ or 2.0%- 4.0% paraformaldehyde in PBS + Mg2+ on ice for 30' to several hours. The amount of time should be determined empirically. Generally, it is wise to minimize time in fixative as the lacZ
solution)to microcentrifuge tube. 2.Add 2.5x 10,000 particles of 1 uM Fluosphere beads. 3.Mix overnight at 4 ℃ with 200 µl 5mg/ml 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. 4.Wash with PBS,by centrifuging,repeat wash 3 times. 5.Resuspend the bead
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
through methanol series (25%, 50%, 75%, 2x 100%) and store in methanol at -20 °C. Take pictures after placing back in methanol. BM purple becomes more blue and intense in methanol.
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