重组新冠病毒 NB.1.8.1 (Omicron) Spike RBD 蛋白 (His & AVI Tag), Biotinylated

重组新冠病毒 NB.1.8.1 (Omicron) Spike RBD 蛋白 (His & AVI Tag), Biotinylated（产品说明）
蛋白名称：SARS-CoV-2 NB.1.8.1 (Omicron) Spike RBD Protein (His & AVI Tag), Biotinylated (HPLC-verified)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (NB.1.8.1) Spike RBD (YP_009724390.1, with mutation I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, A435S, N440K, V445H, G446S, N450D, L452W, L455S, F456L, N460K, S477N, T478I, N481K, del483, E484K, F486P, Q493E, Q498R, N501Y, Y505H) (Arg319-Lys529) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the SARS-CoV-2 variant (known as variant NB.1.8.1).
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性：Immobilized Recombinant SARS-CoV-2 XEC Spike RBD Protein (His & AVI Tag), Biotinylated(Cat: 40592-V49H25-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag)(Cat: 10108-H02H), the EC50 is 2-6 ng/mL (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 319
蛋白分子量：The recombinant SARS-CoV-2 (NB.1.8.1) Spike RBD consists of 236 amino acids and predicts a molecular mass of 27.11 kDa. As a result of glycosylation, it migrates as an approximately 42.2 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-lys529