BioSIM 抗人 P-selectin/CD62 抗体 (

产品核心概述

本产品为BioSIM系列科研级生物类似药，靶向人P-selectin/CD62分子，对应原研药英克西尤单抗（Inclacumab），仅用于基础科研用途，不得用于临床诊断、治疗或人体实验。

• 英文名称：BioSIM anti-Human P-selectin/CD62 Antibody (Inclacumab Biosimilar), Research Grade
• 中文名称：BioSIM 抗人 P-selectin/CD62 抗体 (英克西尤单抗)，生物类似药，科研用途
• CAS号：1256258-86-2
• 产品货号：SIM0221-5mg
• 产品规格：5mg/支
• 产品类型：重组单克隆二抗（人源化）

关键参数详情

生物学属性

• 来源宿主：Human（人源化表达）
• 反应性：Human（特异性识别人源P-selectin/CD62，无交叉反应性验证）
• 免疫原：请参考产品随附说明书（详细免疫原序列/来源需查阅文档）

供应商与质控

• 供应商：InvivoCrown
• 质控标准：符合科研级重组抗体的纯度、活性及内毒素控制要求

生产工艺与背景

本生物类似药遵循原研药英克西尤单抗的关键质量属性（CQA）设计，生产过程具备以下核心特点：

• 生产环境：无动物成分（Animal-Free）洁净设施，避免外源性动物源污染物引入
• 细胞培养：采用体外无蛋白细胞培养技术，减少非特异性蛋白干扰
• 纯化工艺：多步骤纯化流程，核心步骤包含Protein A/G亲和色谱，确保抗体纯度与结合活性

背景提示：P-selectin/CD62为细胞黏附分子，参与炎症、血栓形成等病理过程，是镰状细胞病、心血管疾病研究的关键靶点。

应用场景与研究领域

适用实验类型

• ELISA：人源样本中P-selectin/CD62的定量检测（如血浆、细胞裂解液）
• FACS（流式细胞术）：细胞表面P-selectin表达的定量分析与分选
• Functional Assay（功能实验）：P-selectin介导的细胞黏附、信号通路激活等功能验证
• Research in vivo（体内研究）：动物模型中P-selectin相关病理过程的干预研究

重点研究方向

• 镰状细胞病：红细胞黏附、血管阻塞机制探索
• 心血管疾病：血栓形成、动脉粥样硬化、心肌缺血再灌注损伤等领域

储存与稳定性建议

为维持抗体活性与稳定性，需严格遵循以下储存条件：

• 短期使用（1-2周）：4°C避光保存（避免反复冻融）
• 中期储存（最长12个月）：-20°C避光保存（建议分装为100μL/管，减少冻融次数）
• 长期储存（>12个月）：-80°C避光保存（分装后冻存，避免反复解冻）

注意事项：反复冻融会导致抗体聚合与活性下降，使用前请缓慢解冻（冰浴）并轻轻混匀，避免剧烈震荡。

供应商信息

供应商：InvivoCrown

InvivoCrown专注于科研级重组抗体、生物类似药及生命科学试剂的研发与生产，为全球科研机构提供符合严格质控标准的工具产品。

Product Core Overview

This product is a BioSIM series research-grade biosimilar, targeting the human P-selectin/CD62 molecule, corresponding to the reference drug Inclacumab. It is for basic research use only and not intended for clinical diagnosis, treatment, or human experimentation.

• English Name: BioSIM anti-Human P-selectin/CD62 Antibody (Inclacumab Biosimilar), Research Grade
• Chinese Name: BioSIM Anti-Human P-selectin/CD62 Antibody (Inclacumab), Biosimilar, Research Use
• CAS No.: 1256258-86-2
• Product Cat. No.: SIM0221-5mg
• Product Specification: 5mg/vial
• Product Type: Recombinant Monoclonal Secondary Antibody (Humanized)

Key Parameter Details

Biological Properties

• Host Source: Human (Humanized Expression)
• Reactivity: Human (Specifically recognizes human P-selectin/CD62; no cross-reactivity validation)
• Immunogen: Please refer to the product insert (detailed immunogen sequence/source requires document review)

Supplier & Quality Control

• Supplier: InvivoCrown
• Quality Control Standards: Meet the purity, activity, and endotoxin control requirements for research-grade recombinant antibodies

Production Process & Background

This biosimilar is designed in compliance with the Critical Quality Attributes (CQAs) of the reference drug Inclacumab, with the following core features in the production process:

• Production Environment: Animal-Free clean facility, avoiding introduction of exogenous animal-derived contaminants
• Cell Culture: In vitro protein-free cell culture technology is used to reduce non-specific protein interference
• Purification Process: Multi-step purification procedure, with core steps including Protein A/G affinity chromatography, ensuring antibody purity and binding activity

Background Note: P-selectin/CD62 is a cell adhesion molecule involved in pathological processes such as inflammation and thrombosis, and is a key target in research of sickle cell disease and cardiovascular diseases.

Application Scenarios & Research Fields

Applicable Experimental Types

• ELISA: Quantitative detection of P-selectin/CD62 in human samples (e.g., plasma, cell lysates)
• FACS (Flow Cytometry): Quantitative analysis and sorting of P-selectin expression on cell surfaces
• Functional Assay: Functional validation of P-selectin-mediated cell adhesion, signaling pathway activation, etc.
• Research in vivo: Intervention research on P-selectin-related pathological processes in animal models

Key Research Directions

• Sickle Cell Disease: Exploration of mechanisms of erythrocyte adhesion and vascular occlusion
• Cardiovascular Diseases: Areas such as thrombosis, atherosclerosis, and myocardial ischemia-reperfusion injury

Storage & Stability Recommendations

To maintain antibody activity and stability, the following storage conditions must be strictly followed:

• Short-term use (1-2 weeks): Store at 4°C protected from light (avoid repeated freeze-thaw cycles)
• Medium-term storage (up to 12 months): Store at -20°C protected from light (recommended to aliquot into 100μL/tube to reduce freeze-thaw cycles)
• Long-term storage (>12 months): Store at -80°C protected from light (freeze after aliquoting; avoid repeated thawing)

Notes: Repeated freeze-thaw cycles may cause antibody aggregation and reduced activity. Thaw slowly (on ice) and mix gently before use; avoid vigorous shaking.

Supplier Information

Supplier: InvivoCrown

InvivoCrown focuses on R&D and production of research-grade recombinant antibodies, biosimilars, and life science reagents, providing tool products meeting strict quality control standards for global research institutions.