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- 文献和实验
- 技术资料
- 保存条件:
Store the product under sterile conditions after opening at -80℃ for 12 months. Store the lyophilized powder at -20℃. Avoid repeated freeze-thaw cycles.
- 英文名:
Recombinant Human IGF-II R Protein, C-His Tag
- 库存:
999
- 供应商:
卡梅德生物科技(天津)有限公司
- 规格:
20ug///50ug/// 100ug///200ug///1mg
产品名称:Recombinant Human IGF-II R Protein, C-His Tag
产品编号:KRP167
产品描述:Human IGF2R(43-1365 aa)
分子名称:IGF2R
物种:Human
表达系统:HEK293F Cells
规格:20ug, 50ug, 100ug, 200ug, 1mg
纯度:≥95%
缓冲体系:Supplied in PBS
保存条件:Store the product under sterile conditions after opening at -80℃ for 12 months. Store the lyophilized powder at -20℃. Avoid repeated freeze-thaw cycles.
运输条件:This product is shipped on ice packs or dry ice.
产品声明:该产品仅供科研使用,不可直接用于人体或注射。
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文献和实验干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密
CUT&Tag(Cleavage Under Targets and Tagmentation)是蛋白-DNA 互作的一大革新技术,它不需要使用甲醛交联以及免疫共沉淀,而是通过针对靶蛋白(如转录因子、染色质重塑蛋白)的抗体和 Protein A/G 的介导,使得与 Protein A/G 融合的 Tn5 酶(Tagmentase)在切割 DNA 片段的同时在序列两端加上测序接头,经 PCR 扩增后即可形成用于高通量测序的文库(见图 1 )。CUT &Tag 技术具有细胞投入量低、信噪
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
., Janjua, H., Shastry, R., Ho, C.K., Wang, D., Wang, H., Jiang, M., Montelione, G.T., Stuart, D.I., Owens, R.J., Daenke, S., Schutz, A., Heinemann, U., Yokoyama, S., Bussow, K., and Gunsalus, K.C. 2008. Protein production and purification. Nat. Methods
Experimental Applications: Human Acetylcholinesterase as a Model Nervous System Protein
, 5 ng in vitro-transcribed AChE mRNA directed the production of catalytically active recombinant human acetylcholinesterase (rHAChE), at levels of approx 10-fold above background oocyte levels (Table 6 ). Oocyte-produced rHAChE was sensitive to the AChE
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