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文献和实验Aβ-Induced Proinflammatory Cytokine Release from Differentiated Human THP-1 Monocytes
As noted in the introductory chapters of this book, neuritic plaques composed of accumulated amyloid β (Aβ) peptide are a hallmark pathological feature of the Alzheimer’s disease (AD) brain. Compelling genetic data now implicate these plaques
Screening of compounds toxicity against human Monocytic cell line-THP-1 by flow cytometry
The worldwide rapid increase in bacterial resistance to numerous antibiotics requires on-going development of new drugs to enter the market. As the development of new antibiotics is lengthy and costly, early monitoring of compound’s toxicity
我养过皮肤成纤维细胞和THP-1细胞。皮肤成纤维细胞是贴壁生长的。THP-1细胞是悬浮的。由于我们实验室养细胞的时间也不是很长,所以也只能说说自己平时的操作了。先说皮肤成纤维细胞。当细胞铺满瓶底,也就是细胞与细胞之间没有间隙时,就可以传代了。一般十天左右传一代。先吸弃旧培养液,用D-Hanks液洗两遍,再加入0.25%的胰酶,加入的量以能覆盖瓶底为准。加入后轻轻摇晃培养瓶使液体覆盖瓶底,然后静置两分钟左右,最好是能在显微镜下观察,当细胞之间出现间隙且有一部分细胞呈现圆形后即可。吸去胰酶,加入含
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