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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
HexadecanaMide, N-Methyl-
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
7388-58-1
- 规格:
100mg
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文献和实验Steady State ATPase Assays Coupled Enzyme System
from Rabbit Muscle in 50% glycerol) Procedure 1. Assemble microtubules for assays. E.g., 58 µL of 50 µM MTs = 50 µL 5.8 mg/mL tubulin + 0.5 µL 100 mM Mg・GTP, incubate for 30-60 min at 37°C
Crystallization of Kinesin Family Motor Proteins
temperature11 mg/ml protein 10 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM MgCl2, 0.2 mM NaN3, 2 mM ADP combined 1:1 with 22% methyl ether PEG 2000, 100 mM NaCl, 2% ethylene glycol, 50 mM HEPES pH 7.0Gulick et al., 1998Ncd 281-700Sitting drop 4°C20 mg/ml
BrdU-Immunoperoxidase Staining of Paraffin Tissue Sections
substrate, (make fresh): 25 mL 50 mM Tris pH8, 1 mL DAB (3,3-diaminobenzidine, 25 mg/mL stock, -20ºC), 100 µL NiCl2 (10% w/v stock, 4ºC), 10 µL 30% H2 O2. Counter stains: Methyl Green or dilute Eosin (1/4x in 80% EtOH) BrdU Labeling 1. Inject
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