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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
6-(benzyloxy)-2,3-dihydroinden-1-one
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
25083-80-1
- 规格:
250mg
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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
for annealing / hybridisation, etc.Taq polymerase is given as having a half-life of 30 min at 95℃, which is partly why one should not do more than about 30 amplification cycles: however, it is possible to reduce the denaturation temperature after about 10 rounds
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
1)为了在SG反应后作溶解曲线,引入与溶解曲线起始温度点相同的保温步骤(大约60秒),这样可以确保溶解温度起始于一个确定的温度。2)溶解曲线分析可以重复。可以在相当一段时间内重新作溶解曲线,甚至到第二天也可以。3)关于可以用SG检测的引物对的列表4) 进行SG分析的典型程序是什么?变性:检查反应所用的酶(2min,5min,10min或15min)循环:95度/20秒,退火温度/20秒,72度/20秒保温:72度/60秒溶解:72-99度,on Melt A5)在哪个通道SG可以被检测?双通道
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