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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
−20℃
- 英文名:
MONOCAPRYLIN
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
19670-49-6
- 规格:
100g
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文献和实验for analysis. This reduces the complexity of the peptide mixture before MS is performed and facilitates phosphopeptide identification (Fig. 14). Phosphopeptides can be identified from a mixture of peptides by a method known as precursor ion scanning (116
TOP10 chemically competent cells
of ice cold CCMB80 buffer. Test OD of a mixture of 200 μl SOC and 50 μl of the resuspended cells. Add chilled CCMB80 to yield a final OD of 1.0-1.5 in this test. Incubate on ice for 20 minutes Aliquot to chilled screw top 2 ml
) of 125-I using microsyringe 3. Add 1-2 pieces of IODO-BEADs¶ 4. incubate at room temp, 5 min 5. Add your protein(1-100µg, 25 µg is good for IgGs or Fab) 6. Incubateat room temp, 10-25 min 7. Take reaction mixture (leave beads) and apply
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