LGX 818;1269440-17-6;10mM in D

类器官培养与应用——结直肠类器官

-01 - 500nM - -   N-acetyl-L-cysteine - 1μM - -   Nicotinamide - 10mM - -   Gastrin I - 10nM - -   氯化铵 - - 0.8% -   EDTA - - 0.1mM -   DMSO - - - 10%     组织

λ噬菌体DNA的小量制备

1. Grow a fresh overnight culture of lambda host cells (we use K803 for EMBL3) in LB or T-broth fortified with 10mM Mg++ . 2. Mix a small amount (1 to 20μl, aim for an M.O.I. of +0.01) of high titer phage lysate (e.g. from a plate lysate

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

H20 ChIP elution buffer       20ml final volume:       500µl 2M Tris       2ml 10% SDS       400µl 0.5M EDTA       17.1ml H2O cold lysis buffer       1mM PMSF       20units RNasin       10mM Tris-HCl pH 7.4       10mM NaCl       3mM MgCl2