2,3,4,5,6-PENTAFLUOROBENZHYDRO

蛋白偶联到羧基化微球的方法

minutes.11.Remove the supernatant and resuspend the microspheres in 250 μL of 50 mM MES, pH 5.0 by vortex and sonication for approximately 20 seconds. See Technical Note 1.12.Pellet the microspheres by microcentrifugation at ≥ 8000 x g for 1-2 minutes.13

【信息】Northstar BioProducts（NSBP）

100686-1  O-Glycosidase, (Streptococcus pneumonia, recombinant), EC 3.2.1.97  0.075 unit   60 uL solution in 50 mM Sodium Phosphate pH 7.5     Activity: ≥16 units/mg, ≥17 units/mL. M.W.: ~180,000 (SDS-PAGE)        100688-1  PNGase F, (Chryseobacterium

SOC培养基配方+ITPG

灭菌将Bacto® 胰蛋白胨、Bacto® 酵母抽提物、NaCl 及KCl 加到97ml 的去离子水中，搅拌使之溶解。高压灭菌并冷却到室温。加入2M Mg2+母液和2M 葡萄糖至终浓度20mM。补加消毒蒸馏水至100ml。完全培养基用0.2μm 的滤膜过滤灭菌。最终pH 值为7.0。 2M Mg2＋母液 20.33g MgCl2• 6H2O 24.65g MgSO4• 7H2O 加蒸馏水至100ml，过滤灭菌。