单分散羧基二氧化硅微球;7631-86-9;Carboxyl

原创：western protocol

1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary

Transformation of E. coli by Electroporation

absolute ethanol. Ice 15 min. 3. Centrifuge at >12,000 x g for 15 min at 4° C. Carefully decant the supernatant. 4. Wash the pellet with 1 ml of 70% ethanol. Centrifuge at >12,000 x g for 15min at room temperature. Remove the supernate. 5. Air

Recipe for yeast tryptone medium

stock conc final conc. / 10ml Tris-HCl , pH 7.5 1M 10mM 0.1ml NaCl 5M 50mM 0.1ml b mercapto-ethanol 10mM 7 ml Gelatin 10mg/ml 100 mg/ml 0.1ml water 5.3ml glycerol 44%(v/v) 4.4 ml DNA digestion: restriction enzyme core reaction