单分散羧基二氧化硅微球;7631-86-9;Carboxyl

v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性

supply. 2. Pre-poured SDS-polyacrylamide mini-gels (Bio-Rad or Invitrogen);store refrigerated. 3. Running buffer: 25 mM Tris, 192 mM glycine, and 0.1 %(w/v) SDS. Store at room temperature. 4. Pre-stained molecular mass markers: Kaleidoscope

细胞外囊泡（细胞微粒、外泌体）检测新趋势

对的样本主要是细胞，散射光的检测极限通常是300-500nm，而大多数细胞外囊泡的直径都在300nm以下，由于无法与背景噪音区分，直径小于300nm的细胞外囊泡很难被检测到，因此，囊泡数量往往被低估，检测结果自然也不准确[1]。 Apogee流式细胞仪的三大优势 （1）最灵敏的散射光分辨

原创：western protocol

1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary