ML380;1627138-52-6;10mM in DMS

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

H20 ChIP elution buffer       20ml final volume:       500µl 2M Tris       2ml 10% SDS       400µl 0.5M EDTA       17.1ml H2O cold lysis buffer       1mM PMSF       20units RNasin       10mM Tris-HCl pH 7.4       10mM NaCl       3mM MgCl2      

类器官培养与应用——结直肠类器官

RNase-Free DNase 30 Kunitz Units - - -   GlutaMAX - 2mM - -   Non-Essential Amino Acids - 1× - -   HEPES - 10mM - -   N2 - 1× - -   B27 - 1× - -   Heparin - 4μg/ml

In Situ HYBRIDIZATION TO TISSUE SECTIONS

1.0ml 10mM rGTP 1.0ml 0.5mM rUTP 1.0ml 0.75M DTT 1.0ml RNase-Block (25U/ml) 2.5ml 35S-rUTP (100mCi) 1.0ml T3 or T7 polymerase (10U) Vortex, spin down and incubate 1-2h @ 30 degrees C. Incorporation should be 70-90%. Determine