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- 源叶
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- T93867-1ml
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80℃（运输条件：冰袋低温运输）
- 英文名：
(20S)-Protopanaxatriol
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- CAS号：
34080-08-5
- 规格：
1ml
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文献和实验Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes
Proteasomes are the main cytosolic proteases responsible for generating peptides for antigen processing and presentation in the MHC (major histocompatibility complex) class-I pathway. Purified 20S and 26S proteasomes have been widely used
Phosphoamino acid analysis:Mark Kamps's method
more than 3 µl is tedious,so don't use much H20.On the other hand,I wouldn't use much less than 6 µl so as to have good recovery. 7.Spot 1 µl PAA standards (about 0.3 µg each of PSer,PThr,and PTyr)on a cellulose thin layer chromatography plate and then spot
Co-staining with Thioflavin S & Carboxyfluorescein
Co-staining with Thioflavin S & Carboxyfluorescein 1.) Prepare 0.01% thioflavin S (T1892 , Sigma) solution in F12+ [0.01g / 100 ml F12+] 2.) Filter the staining solution with a 0.22 µm Millipore filter
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