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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 英文名:
WAY-332035
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
1015858-20-4
- 规格:
5mg
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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
) through the oil to sample. (It's 0.3 ul per 15ul original volume, so adjust as necessary). Incubate overnight at 55 C. Day 3: (Repeat:) Add 0.3 ul fresh conc Proteinase K (20 mg/ml stock) through the oil to sample. (It's 0.3 ul per 15 ul original
Single tube confirmation PCR protocol
. For example, add 5 µl of the Zymo solution from isolate #1 to PCR tubes 1-5, 5 µl of the Zymo solution from isolate #2 to PCR tubes 6-10 and so on. - Make a PCR master mix by combining: 638 µl water, 110 µl 10 x Taq Buffer, 11 µl NTP's, and 11 µl Taq
Co. 07596-20 Pump-Drive (Masterflex L/S variable-speed economy drive) Cole Parmer Instrument Co. 7524-40
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